B. D. Freudenthal

Biochemical and Structural Characterization of Fapy·dG Replication by Human DNA Polymerase β

This study investigates how human DNA polymerase β processes the mutagenic lesion Fapy*dG during DNA replication, showing inefficient bypass and incorporation, suggesting mutagenicity arises from bypass rather than lesion incorporation.

Single-molecule analysis of purified proteins and nuclear extracts: insights from 8-oxoguanine glycosylase 1

This study compares single-molecule DNA binding dynamics of purified OGG1 and nuclear extracts using SMADNE, revealing differences in undamaged DNA binding and increased binding lifetime for a catalytically dead variant in nuclear extracts.

Single-molecule analysis reveals TDG exhibits multiple modes of linear diffusion to process 5-formylcytosine.

This study uses single-molecule fluorescence to show TDG uses multiple diffusion modes to search for DNA lesions and how its structure affects nucleosome interactions.

The VEGF G-quadruplex forming promoter is repaired via long-patch BER

The paper investigates how BER enzymes repair oxidative damage in the VEGF G4 promoter, revealing that long-patch BER is required due to multiple insertions by Pol β.

Visualizing the Coordination of APE1 and DNA Polymerase β During Base Excision Repair

This study uses single-molecule microscopy to visualize the coordination of APE1 and DNA polymerase β during base excision repair, showing that intermediate transfer depends on enzyme dissociation kinetics.

Structural basis for APE1 processing DNA damage in the nucleosome

This study reveals how APE1 processes DNA damage in nucleosomes by bending DNA to access AP sites, with efficiency depending on site exposure.

Mechanism of nucleotide discrimination by the translesion synthesis polymerase Rev1

This study investigates how Rev1 discriminates between nucleotides during translesion synthesis, showing structural differences in hydrogen bonding and sugar pucker when using dCTP versus rCTP.

Processing of oxidatively damaged DNA dirty ends by APE1

This study characterizes APE1's exo activity on 3-8-oxoG substrates, revealing a unified mechanism distinct from its AP-endonuclease activity and identifying unique protein:DNA contacts for 8-oxoG removal.

Construction of a 3-color prism-based TIRF microscope to study the interactions and dynamics of macromolecules

The paper describes the construction of a 3-color prism-based TIRF microscope for studying macromolecular interactions and dynamics.

Anthrax Toxin Translocation Complex Reveals insight into the Lethal Factor Unfolding and Refolding mechanism

This paper reports a cryoEM structure of the anthrax toxin pore complex showing lethal factor unfolding and refolding during translocation.

Visualizing Rev1 Catalyze Protein-template DNA Synthesis

This study uses time-lapse X-ray crystallography to visualize Rev1's mechanism of catalyzing protein-template DNA synthesis, revealing key steps in nucleotide incorporation and product release.