M. Spies

The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA.

This study shows the Shu complex enhances RAD51 activity by stabilizing filaments and promoting RPA diffusion on ssDNA, with implications for cancer therapy.

Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair

This study generates a fluorescently labeled human XPA protein to study DNA binding dynamics during nucleotide excision repair.

A double-ring of human RAD52 remodels replication forks restricting fork reversal

The paper shows human RAD52 forms a double-ring structure around stalled replication forks, remodeling them via DNA strand exchange to prevent reversal by SMARCAL1.

Small molecule nitroalkenes inhibit RAD51-mediated homologous recombination and amplify triple-negative breast cancer cell killing by DNA-directed therapies.

This paper identifies nitroalkenes that inhibit RAD51-mediated homologous recombination, enhancing DNA damage in triple-negative breast cancer cells and showing potential as anticancer agents.

The distinct roles of MSH2 and MLH1 in basal-like breast cancer and immune modulation

This study identifies distinct roles of MSH2 and MLH1 in basal-like breast cancer, showing MSH2 promotes metastasis while MLH1 enhances it, and reveals MSH2's role in immune modulation through IFNAR1 regulation.

Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon χ recognition

The paper investigates how RecBn loads RecA onto ssDNA via a χ-dependent mechanism, showing that RecBn can function independently of its flexible linker.

Human hnRNPA1 reorganizes telomere-bound Replication Protein A

The paper shows hnRNPA1 remodels RPA bound to telomeric ssDNA, forming a ternary complex regulated by TERRA.

Single-molecule analysis of the improved variants of the G-quadruplex recognition protein G4P

The paper analyzes the binding kinetics and selectivity of G4P and its variants using single-molecule techniques, finding that increasing RSM units enhances affinity for telomeric G4s.

RAD52 prevents accumulation of Polα-dependent replication gaps at perturbed replication forks

This study shows RAD52 prevents Polα-dependent replication gaps by enabling RAD51/Pol repriming at reversed replication forks, which limits DNA damage and improves cell viability during replication stress.

Construction of a 3-color prism-based TIRF microscope to study the interactions and dynamics of macromolecules

The paper describes the construction of a 3-color prism-based TIRF microscope for studying macromolecular interactions and dynamics.

KERA: Analysis Tool for Multi-Process, Multi-State Single-Molecule Data

KERA is a software tool that analyzes single-molecule data to identify interaction sequences and dwell times, providing insights into molecular complex dynamics.

Dynamics and Selective Remodeling of the DNA Binding Domains of RPA

The paper visualizes RPA's DNA binding domain dynamics and shows how Rad52 modulates DBD-D to enable ssDNA remodeling during homologous recombination.