C. L. Lei

The impact of uncertainty in hERG binding mechanism on in silico predictions of drug-induced proarrhythmic risk

This study evaluates how uncertainty in hERG binding mechanisms affects in silico predictions of drug-induced proarrhythmic risk.

Leak current, even with gigaohm seals, can cause misinterpretation of stem cell-derived cardiomyocyte action potential recordings

This paper shows seal-leak current affects iPSC-CM action potential morphology even with high seal resistance, and suggests ways to improve data interpretation and modeling.

Accounting for variability in ion current recordings using a mathematical model of artefacts in voltage-clamp experiments

This paper develops a mathematical model to explain variability in patch-clamp data by accounting for experimental artefacts rather than cell-specific ion channel kinetics.

Rapid characterisation of hERG channel kinetics II: temperature dependence

This paper characterizes the temperature dependence of hERG channel kinetics using a high-throughput protocol and finds that Q10 coefficients poorly represent the temperature sensitivity, while a generalized Eyring relationship fits better.

Rapid characterisation of hERG channel kinetics I: using an automated high-throughput system

This paper introduces a 15-second high-throughput protocol to rapidly characterize hERG channel kinetics using automated patch clamp systems and validates it with a hierarchical Bayesian model.