Mechanisms of improved specificity of engineered Cas9s revealed by single molecule analysis
D. Singh, Y. Wang, J. Mallon, O. Yang, J. Fei, A. Poddar, D. Ceylan, S. Bailey, T. Ha
This study reveals how engineered Cas9 variants improve specificity by altering DNA unwinding dynamics and cleavage rates in response to mismatches.
bioRxiv · Sep 22, 2017Biophysics