J. Fei

Transcriptome-wide mRNP condensation precedes stress granuleformation and excludes new mRNAs

The paper shows that mRNAs condense broadly in yeast under stress, independent of length, and new transcripts escape this condensation for selective translation.

Dynamics of RNA localization to nuclear speckles are connected to splicing efficiency

The paper investigates RNA localization to nuclear speckles and finds that splicing efficiency is linked to transcript enrichment in these organelles.

Translational T-box riboswitches bind tRNA by modulating conformational flexibility

This study uses single-molecule FRET to analyze the binding mechanism of the Mycobacterium tuberculosis IleS T-box riboswitch, revealing a two-step model and the role of specific sequences in conformational flexibility and tRNA recognition.

Comparative genetic, biochemical, and biophysical analyses of the four E. coli ABCF paralogs support distinct functions related to mRNA translation

The paper compares four E. coli ABCF paralogs to determine their distinct roles in mRNA translation using biochemical and biophysical assays.

RNA molecules display distinctive organization at nuclear speckles

The paper uses super-resolution imaging to show that different RNA sequence domains localize to distinct regions within nuclear speckles, driven by RNA-protein interactions.

Entropic control of the free energy landscape of an archetypal biomolecular machine

This study reveals how ligands modulate the free energy landscape of the bacterial ribosome by altering its structural flexibility, suggesting entropic control as a general mechanism for regulating biomolecular machines.

Skeletal muscle lineage is dispensable for appendage regeneration in axolotl

This study shows that skeletal muscle is not required for limb regeneration in axolotls, as Pax7 mutants regenerate limbs with normal patterns and cell composition.

Kinetic model of small RNA-mediated regulation suggests that a small RNA can regulate co-transcriptionally

The paper develops a kinetic model to study sRNA-mediated gene regulation, revealing co-transcriptional regulation and identifying binding as a rate-limiting step.

K29-Linked Ubiquitin Signaling Regulates Proteotoxic Stress Response and Cell Cycle

This study identifies sAB-K29, a tool to detect K29-linked polyubiquitin, and shows its role in proteotoxic stress response and cell cycle regulation.

Deoxyribozyme-based Method for Site-specific Absolute Quantification of N6-methyladenosine Modification Fraction

This paper introduces a deoxyribozyme-based method to absolutely quantify m6A modification fractions at specific RNA sites using reverse transcription and qPCR.

Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs and mRNAs in live bacterial cells

This study uses live-cell imaging to show Hfq dynamically interacts with mRNAs and sRNAs, recruiting RNase E to promote mRNA turnover and enabling sRNA-mediated gene regulation.

Two-step binding kinetics of tRNAGly by the glyQS T-box riboswitch and its regulation by T-box structural elements

The paper investigates the two-step binding kinetics of tRNAGly by the glyQS T-box riboswitch and identifies structural elements that regulate each step.

Mechanisms of improved specificity of engineered Cas9s revealed by single molecule analysis

This study reveals how engineered Cas9 variants improve specificity by altering DNA unwinding dynamics and cleavage rates in response to mismatches.