A. Poddar

da_Tracker: Automated workflow for high throughput single cell and single phagosome tracking in infected cells.

The paper introduces da_Tracker, an automated workflow for high-throughput single-cell and single-phagosome tracking in infected cells, enabling quantitative analysis of pathogen-host interactions.

The mechanosensitive Pkd2 channel modulates the recruitment of myosin II and actin to the cytokinetic contractile ring

This study shows that the Pkd2 channel regulates myosin II and actin recruitment to the cytokinetic contractile ring in fission yeast, suggesting a calcium-dependent mechanism.

GRK2 Kinases in the Primary Cilium Initiate SMOOTHENED-PKA Signaling in the Hedgehog Cascade

This study identifies GRK2-mediated phosphorylation of SMO in the primary cilium as a critical step initiating SMO-PKA signaling in the Hedgehog pathway.

Dynamic Interplay of Autophagy and Membrane Repair During Mycobacterium tuberculosis Infection

This study investigates LC3 recruitment to Mtb-containing vacuoles in macrophages, revealing its variability and lack of correlation with autophagosome maturation or membrane repair.

Membrane stretching activates calcium-permeability of a putative channel Pkd2 during fission yeast cytokinesis

This study shows that Pkd2, a putative ion channel in fission yeast, allows calcium influx when activated by membrane stretching during cytokinesis.

Calcium spikes accompany the cleavage furrow ingression and cell separation during fission yeast cytokinesis

The paper identifies calcium spikes during fission yeast cytokinesis and shows they promote contractile ring constriction and daughter cell integrity.

Cocculus hirsutus -derived phytopharmaceutical drug has potent anti-dengue activity

This paper identifies Cocculus hirsutus-derived AQCH as a potent anti-dengue drug with in vitro and in vivo efficacy, characterized by five chemical markers and formulated into tablets for further development.

Stochastic Analysis Demonstrates the Dual Role of Hfq in Chaperoning E. coli Sugar Shock Response

The paper uses single-cell microscopy and stochastic modeling to show Hfq stabilizes SgrS and aids its interaction with target mRNA during glucose-phosphate stress in E. coli.

Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis

This study identifies Pkd2p, a TRP channel in fission yeast, as a regulator of cytokinesis by modulating osmotic homeostasis and cell separation.

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1

This study characterizes the DNA interrogation and product release kinetics of three Cpf1 orthologues using single-molecule fluorescence and biochemical assays, revealing differences from Cas9 in binding and cleavage requirements.

Determinants of target prioritization and regulatory hierarchy for the bacterial small RNA SgrS

This study investigates how the bacterial sRNA SgrS prioritizes and regulates its target mRNAs, revealing that regulatory hierarchy depends on binding site number, position, and cofactors like Hfq.

Mechanisms of improved specificity of engineered Cas9s revealed by single molecule analysis

This study reveals how engineered Cas9 variants improve specificity by altering DNA unwinding dynamics and cleavage rates in response to mismatches.