D. Ermolenko

The paper uses two FRET assays to study intersubunit rotation in bacterial ribosomes, finding spontaneous rotation occurs without EF-G and GTP hydrolysis.

The paper shows that specific length and structure, not high thermodynamic stability, of mRNA stem-loops cause ribosome pauses by inhibiting tRNA binding and slowing translocation.

This paper explores the biological implications of mRNA end proximity caused by secondary structure, suggesting roles in translation initiation and coupling of deadenylation to mRNA decay.

The paper shows that increasing the spacer length between the Shine-Dalgarno sequence and P-site codon reduces mRNA translocation rate by 5-10 fold.

The paper investigates how mRNA stem-loops impede ribosome movement by hindering A-site tRNA binding and rotation during translation elongation.