D. M. Fowler

A mutational atlas for Parkin proteostasis

This study maps Parkin missense variants' abundance and degradation mechanisms, showing most low-abundance variants are degraded via the ubiquitin-proteasome system and stabilized at lower temperatures.

Image-based identification and isolation of micronucleated cells to dissect cellular consequences

This study develops two MN segmentation tools to quantify micronuclei and analyze their transcriptomic effects in human cells.

Opto-MASS: a high-throughput engineering platform for genetically encoded fluorescentsensors enabling all optical in vivo detection of monoamines and neuropeptides

The paper introduces Opto-MASS, a high-throughput platform for engineering fluorescent sensors to detect monoamines and neuropeptides in vivo by screening thousands of variants in mammalian cells.

Conservation of endangered galaxiid fishes in the Falkland Islands requires urgent action on invasive brown trout

The paper assesses the impact of invasive brown trout on native galaxiid fishes in the Falkland Islands, finding high establishment success and predicting near-complete invasion within 70 years.

Environmental selection and epistasis in an empirical phenotype-environment-fitness landscape

The paper constructs an empirical phenotype-fitness landscape for VIM-2 β-lactamase across ampicillin concentrations, showing environmental dependence of mutational effects and nonspecific epistasis.

The biophysical basis of protein domain compatibility

The paper uses high-throughput assays and machine learning to model biophysical interactions governing protein domain compatibility in fusion proteins.

Understanding the origins of loss of protein function by analyzing the effects of thousands of variants on activity and abundance

The paper analyzes variant effects on protein activity and abundance in NUDT15 and PTEN, finding that a third of variants cause loss of function and half of these also reduce abundance.

Comprehensive exploration of the translocation, stability and substrate recognition requirements in VIM-2 lactamase.

This study uses deep mutational scanning to analyze VIM-2 lactamase variants, identifying residues affecting stability, translocation, and substrate specificity across different temperatures.

Classifying disease-associated variants using measures of protein activity and stability

The paper classifies PTEN missense variants as pathogenic or benign using experimental and computational methods, finding that ~60% of pathogenic variants destabilize the protein.