J. D. Lee

Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium Indicators.

This study uses machine learning to predict and engineer improved fluorescent calcium indicators, identifying variants with faster kinetics and larger responses.

Far-red and sensitive sensor for monitoring real-time H2O2 dynamics with subcellular resolution and inmulti-parametric imaging applications.

This paper introduces a new fluorescent sensor, oROS-HT, for real-time H2O2 monitoring with subcellular resolution and multi-parametric imaging capabilities.

Structure-guided engineering of a fast genetically encoded sensor for real-time H2O2 monitoring.

This paper develops a fast, sensitive genetically encoded H2O2 sensor (oROS-G) and demonstrates its use in monitoring H2O2 dynamics in various cell types and models.

Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

This study shows that SARS-CoV-2 variants differ in syncytia formation, which affects host chromatin accessibility, cellular senescence, and inflammation via TP53.

Opto-MASS: a high-throughput engineering platform for genetically encoded fluorescentsensors enabling all optical in vivo detection of monoamines and neuropeptides

The paper introduces Opto-MASS, a high-throughput platform for engineering fluorescent sensors to detect monoamines and neuropeptides in vivo by screening thousands of variants in mammalian cells.

SARS-CoV-2 triggers complement activation through interactions with heparan sulfate

This study shows SARS-CoV-2 activates the complement system via heparan sulfate interactions, leading to C5a production and delayed granulocyte/monocyte activation.

Glucose clearance and uptake is increased in the SOD1G93A mouse model of amyotrophic lateral sclerosis through an insulin-independent mechanism

This study shows that SOD1G93A mice exhibit increased glucose uptake and altered glucose homeostasis through an insulin-independent mechanism and impaired glucagon signaling in ALS.

The 'C3aR antagonist' SB290157 is a partial C5aR2 agonist

This paper reveals that SB290157, previously thought to be a C3aR antagonist, acts as a partial agonist at C5aR2 and has mixed effects on C3aR signaling.

Hematopoietic p53 loss cell-extrinsically defines an immune infiltrated microenvironment in leukemia and pre-leukemia

This study shows that loss of hematopoietic p53 leads to immune infiltration in leukemia and pre-leukemia, suggesting immunotherapy potential for TP53-mutated AML.