Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania
C. Asencio, P. Herve, P. Morand…·E. Tetaud
This paper demonstrates a marker-free gene editing method using SpCas9 ribonucleoprotein complexes in trypanosomatids, enabling efficient genetic modifications in various life stages of T. brucei, T. congolense, and Leishmania promastigotes.