G. Dong

The C-terminus of CFAP410 forms a tetrameric helical bundle that is essential for its localization to the basal body

This study determines the structure of CFAP410's C-terminal domain and shows that its tetrameric helical bundle is essential for basal body localization, explaining how the L224P mutation causes ciliopathies.

A novel plasmid-based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells

This paper introduces a yeast-based system allowing the co-expression of up to 20 different proteins by maintaining 10 plasmids in auxotrophic {Delta}10 strains.

Soft and stretchable organic bioelectronics for continuous intra-operative neurophysiological monitoring during microsurgery

This paper introduces a soft, stretchable organic bioelectronic system for continuous intra-operative neurophysiological monitoring during microsurgery, demonstrating improved signal quality and reduced post-operative complications in animal models.

CFAP410 has a bimodular architecture with a conserved surface patch on its N-terminal LRR motif for binding interaction partners

This study reports the crystal structure of the N-terminal domain of CFAP410 and shows how mutations in this domain disrupt its structural stability and interaction with partners, leading to disease.

IGF-1 therapy improves muscle size and function in experimental peripheral arterial disease

This study shows that IGF-1 therapy increases muscle size and strength in a rodent PAD model, with greater effects in female mice.

Characterisation of TbSmee1 indicates that endocytosis is required for access of surface-bound cargo to the trypanosome flagellar pocket

This study characterizes TbSmee1 in Trypanosoma brucei, showing that endocytosis is required for surface-bound cargo to access the flagellar pocket.

Double NPY motifs at the N-terminus of Sso2 synergistically bind Sec3 to promote membrane fusion

This study identifies dual-site interactions between Sso2 and Sec3 that drive secretory vesicle fusion in yeast.

Functional stemness-related genes revealed by single-cell profiling of naïve and stimulated human CD34+ cells from CB and mPB

This study identifies stemness-related genes in human CD34+ cells from different sources and culture conditions using scRNA-seq, revealing pathways involved in stemness maintenance and potential drug targets for HSC expansion.

Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar

This study identifies BILBO2 as a new FPC component and characterizes its interaction with BILBO1 and FPC4, revealing a tripartite complex structure and functional domains.

Discovery of a Potent and Dual-Selective Bisubstrate Inhibitor for Protein Arginine Methyltransferase 4/5

The paper designs and synthesizes a potent dual-selective bisubstrate inhibitor for PRMT4/5, demonstrating high potency and selectivity with IC50 values of 2.8 nM and <1.5 nM.

Restoration of β-globin expression with optimally designed lentiviral vector for β-thalassemia treatment in Chinese patients

This paper develops a lentiviral vector for β-thalassemia treatment that restores β-globin expression in Chinese patients with minimal tumorigenesis risk.

Transcriptome analyses of β-thalassemia -28 (A>G) mutation using isogenic cell models generated by CRISPR/Cas9 and asymmetric single-stranded oligodeoxynucleotides (assODN)

This study uses CRISPR/Cas9 and assODN to create an isogenic cell model of the HBB -28 (A>G) mutation and analyzes its transcriptome effects, revealing altered gene expression and pathway activation.

Probing the Plasticity in the Active Site of Protein N-terminal Methyltransferase 1 Using Bisubstrate Analogs

This study examines bisubstrate analog interactions with NTMT1 to understand active site plasticity and identifies a 4-C linker analog as the most potent inhibitor.

Selective Peptidomimetic Inhibitors of NTMT1/2: Rational design, synthesis, characterization, and crystallographic studies

This paper designs and characterizes peptidomimetic inhibitors of NTMT1/2, showing high selectivity and cell-permeable activity.

Crystal structure of the TbBILBO1 N-terminal domain reveals a ubiquitin fold with a long rigid loop for the binding of its partner

The paper determines the crystal structure of TbBILBO1 N-terminal domain, identifies a hydrophobic pocket for FPC4 binding, and suggests potential drug design targets.

Serum-free culture system for spontaneous human mesenchymal stem cell spheroids formation

This study develops a serum-free method to generate human mesenchymal stem cell spheroids using knockout serum replacement, showing similar transcriptomes to FBS-based spheroids and retained MSC markers.

Structure of a novel dimeric SET domain methyltransferase that regulates cell motility

This paper reports the crystal structure of T. gondii AKMT, revealing a unique dimerization interface and distinct structural features that define a new subclass of KMTs.

Functional analyses of the CIF1-CIF2 complex in Trypanosoma brucei identify the structural motifs required for complex formation and cytokinesis

The paper identifies structural motifs in CIF1 and CIF2 required for complex formation and cytokinesis in Trypanosoma brucei.