Author

E. Pyle

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 6

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:protein folding & dynamics· 3 Organism:bacteria (other)· 2 Assay:cryo-EM· 2 Research Focus:protein structure prediction· 2 Assay:proteomics· 2 Approach:Bayesian inference· 1 Research Focus:benchmarking· 1

Frequent Co-Authors

A. Politis· 3 shared G. Zanetti· 2 shared I. Collinson· 2 shared J. Zivanov· 2 shared R. A. Corey· 2 shared S. Scheres· 2 shared W. J. Allen· 2 shared A. Burt· 1 shared

Activity by Year

2017-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 14 mutual co-author links

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3 shared papersBiophysics

A. Politis

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6 papers

Recent·Most cited

An image processing pipeline for electron cryo-tomography in RELION-5

A. Burt, B. Toader, R. Warshamanage, A. von Kugelgen, E. Pyle, J. Zivanov, D. Kimanius, T. A. M. Bharat, S. Scheres

The paper introduces an image processing pipeline for electron cryo-tomography in RELION-5, enabling automated atomic model building from tomographic data.

bioRxiv · Apr 26, 2024Biophysics

Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes

E. Pyle, E. A. Miller, G. Zanetti

This study uses cryo-electron tomography to show COPII forms spherical vesicles by binding cargo on native membranes, revealing its structure and how it generates membrane curvature.

bioRxiv · Jan 17, 2024Biophysics

A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0

J. Zivanov, J. Oton, Z. Ke, A. von Kügelgen, E. Pyle, K. Qu, D. Morado, D. Castano-Diez, G. Zanetti, T. Bharat, J. Briggs, S. Scheres

The paper introduces a Bayesian method for improving cryo-ET data analysis in RELION-4.0, enabling higher-resolution structure determination from multiple particles.

bioRxiv · Feb 28, 2022Biophysics

HDX-MS reveals nucleotide-regulated, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

Z. Ahdash, E. Pyle, W. J. Allen, R. A. Corey, I. Collinson, A. Politis

This study uses HDX-MS to show that ATP binding causes SecY to open and SecA to close, while ATP hydrolysis reverses this, supporting a Brownian ratchet mechanism for protein translocation.

bioRxiv · Apr 1, 2019Biophysics

Specific cardiolipin-SecY interactions are required for proton-motive-force stimulation of protein secretion

R. A. Corey, E. Pyle, W. J. Allen, M. Casiraghi, B. Miroux, I. Arechaga, A. Politis, I. Collinson

This study identifies and validates two conserved cardiolipin binding sites on the SecA-SecYEG complex, showing their role in ATP and PMF-driven protein secretion.

bioRxiv · Dec 6, 2017Biophysics

Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA

E. Pyle, A. C. Kalli, S. Amillis, Z. Hall, A. C. Hanyaloglu, G. Diallinas, B. Byrne, A. Politis

The paper investigates how membrane lipids affect the structure and function of the purine transporter UapA, showing that specific lipids stabilize its dimeric form and transport activity.

bioRxiv · Oct 20, 2017Biophysics