Fast and efficient generation of knock-in human organoids using homology-independent CRISPR/Cas9 precision genome editing
B. Artegiani, D. Hendriks, J. Beumer, R. Kok, X. Zheng, I. Joore, S. Chuva de Sousa Lopes, J. van Zon, S. Tans, H. Clevers
This paper introduces CRISPR-HOT, a method for efficient knock-in of exogenous DNA in human organoids without requiring homology templates or TP53 inactivation, and demonstrates its application in studying hepatocyte division and ploidy.
bioRxiv · Jan 15, 2020Cell Biology