Kadie A. Holsinger

The paper evaluates how structural modifications like macrocyclization and N-alkylation affect peptide accumulation in Mycobacterium tuberculosis using a novel assay, showing macrocyclization generally enhances accumulation while N-alkylation has context-dependent effects.

This paper introduces a luciferase-based assay to measure antibiotic permeability into the cytosol of Gram-negative bacteria and demonstrates its ability to track intracellular accumulation in real time.