Impact of CRISPR/HDR-editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
B.-C. Lee, A. Gin, C. Wu, K. Singh, M. Grice, R. Mortlock, D. Abraham, X. Fan, Y. Zhou, A. AlJanahi, U. Choi, S. S. de Ravin, T. Shin, S. Hong, C. Dunbar
This study compares CRISPR/HDR and lentiviral transduction effects on HSPC engraftment and clonal dynamics in rhesus macaques, finding HDR-edited cells decline rapidly while lentiviral cells persist longer.
bioRxiv · Dec 13, 2023Cell Biology