X. Fan

A Combinatorial Strategy for HRV 3C Protease Engineering to Achieve the N-terminal Free Cleavage

This paper develops a combinatorial strategy to engineer HRV 3C protease for N-terminal free cleavage by expanding P1 specificity through structure-guided library design and high-throughput screening.

Whole-genome resequencing revealed the Origin and Domestication of Chinese Domestic Rabbits

This study uses whole-genome resequencing to trace the origin and domestication of Chinese domestic rabbits, identifying genetic adaptations and multiple potential origins.

Deep learning based genomic breeding of pest-resistant grapevine

This study uses deep learning and genome-wide association studies to identify pest-resistant genes in grapevine, achieving high accuracy in predicting pest damage.

Multivalent 28S rRNA Is the Organizer of the Nucleolus's Multi-layered Architecture

This study identifies 28S rRNA as a key organizer of the tripartite nucleolus structure through multivalent interactions that drive the formation of the three-layered architecture.

Sec23IP recruits the Cohen syndrome factor VPS13B/COH1 to ER exit site-Golgiinterface for tubular ERGIC formation

This study identifies Sec23IP as a VPS13B adaptor that recruits VPS13B to ER exit sites-Golgi interfaces, promoting tubular ERGIC formation and affecting procollagen export, linking it to Cohen syndrome pathogenesis.

Thymidine Phosphorylase Promotes the Formation of Abdominal Aortic Aneurysm in Mice Fed a Western Diet

This study shows that TYMP promotes abdominal aortic aneurysm (AAA) in mice fed a Western diet by enhancing inflammation, thrombosis, and MMP2 activation while inhibiting vascular smooth muscle cell function.

Restricting CAR T Cell Trafficking Expands Targetable Antigen Space

The paper shows that modifying integrin-mediated adhesion in CAR T cells prevents CNS targeting while maintaining anti-tumor activity against Ewing sarcoma.

Human Amniotic Epithelial Cells Promote Chx10-/Pax6+ Muller Glia Subpopulation Reprogramming into Photoreceptor-like Cells

This study identifies a specific Muller glia subpopulation that can be reprogrammed into photoreceptor-like cells through transplantation of human amniotic epithelial cells.

Impact of CRISPR/HDR-editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques

This study compares CRISPR/HDR and lentiviral transduction effects on HSPC engraftment and clonal dynamics in rhesus macaques, finding HDR-edited cells decline rapidly while lentiviral cells persist longer.

RAD-seq unravels local adaptation along a latitudinal gradient for the wild rice Zizania latifolia

This study uses RAD-seq to analyze genetic adaptation in Zizania latifolia across China's eco-geographical regions, finding environmental factors strongly influence genetic structure and local adaptation.

Tropism of AAV.CPP.16 in the respiratory tract and its application for a CRISPR-based gene therapy against SARS-CoV-2

This study evaluates AAV.CPP.16 as a gene delivery vector for respiratory diseases and demonstrates its use in a CRISPR-based therapy targeting SARS-CoV-2.

Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation

This study shows PthA4 induces CsLOB1, which in turn regulates Cs9g12620 expression, promoting citrus canker through dynamic gene regulation.

Structural basis for VLDLR recognition by eastern equine encephalitis virus

This study determines the structures of EEEV and SFV spike glycoproteins bound to VLDLR, revealing distinct binding modes that explain how different alphaviruses use the same receptor.

Latitude-Driven Functional Trait Variations in Zizania latifolia: Insights into Climate Adaptation

This study transplants Zizania latifolia populations across latitudes to assess climate adaptation, finding high-latitude populations grow smaller and low-latitude populations lack sexual reproduction, suggesting limited adaptability to global warming.

Protein language models-assisted engineering of Uracil-N glycosylase enables programmable T-to-G and T-to-C base editing

This study uses protein language models to engineer a uracil-N-glycosylase variant for programmable T-to-G and T-to-C base editing, achieving high success rates in correcting genetic mutations in mice.

Olfactory learning in Drosophila requires O-GlcNAcylation of mushroom body ribosomal subunits

This study identifies O-GlcNAcylation of mushroom body ribosomal subunits as essential for olfactory learning in Drosophila, showing that reducing O-GlcNAcylation impairs learning.

Urban non-point source pollutants cause microbial community homogenization via increasing deterministic processes

This study shows urban non-point source pollutants homogenize microbial communities by increasing deterministic processes and reducing habitat heterogeneity.

Duhuo Jisheng Decoction Regulates Intracellular Zinc Homeostasis by Enhancing Autophagy via PTEN/Akt/mTOR to Improve Knee Cartilage Degeneration

This study investigates how Duhuo Jisheng Decoction protects cartilage by enhancing autophagy through the PTEN/Akt/mTOR pathway and regulating zinc homeostasis.

Chimeric oncolytic adenovirus evades neutralizing antibodies from human patients and exhibits enhanced anti-glioma efficacy in immunized mice

This paper develops a chimeric oncolytic adenovirus that evades neutralizing antibodies and shows improved anti-glioma efficacy in mice.

Small RNA Sequencing Reveals a Distinct MicroRNA Signature between Glucocorticoid Responder and Glucocorticoid Non-responder Primary Human Trabecular Meshwork Cells after Dexamethasone Treatment

Suppression of tumor cell lactate-generating signaling pathways eradicates murine PTEN/p53-deficient aggressive-variant prostate cancer via macrophage phagocytosis

This study shows that blocking lactate-producing pathways in PTEN/p53-deficient prostate cancer enhances macrophage phagocytosis, leading to durable tumor control in mice.

An isoform-resolution transcriptomic atlas of colorectal cancer from long-read single-cell sequencing

This study builds an isoform-resolution transcriptomic atlas of colorectal cancer using long-read single-cell sequencing and identifies neoepitopes for vaccine development.

Bioorthogonal photocatalytic quinone methide decaging for cell-cell interaction labeling

The paper introduces a method to label and profile cell-cell interactions with high sensitivity and spatial-temporal resolution using a bioorthogonal photocatalytic quinone methide decaging strategy.

Two teosintes made modern maize

This study clarifies the origin of modern maize by showing it resulted from an ancient admixture between domesticated maize and Zea mays ssp. mexicana around 5300 years ago, contributing to genetic and phenotypic diversity.

Abnormal expression of Prolyl oligopeptidase (POP) and its catalytic products Ac-SDKP contributes to the ovarian fibrosis change in Polycystic ovary syndrome (PCOS) mice

This study shows that reduced Prolyl oligopeptidase (POP) and its product Ac-SDKP contribute to ovarian fibrosis in PCOS mice by altering MMP-2 and TGF-β1 expression.

Integrated transcriptome and lineage analyses reveal novel catecholaminergic cardiomyocytes contributing to the cardiac conduction system in murine heart

This study identifies a novel catecholaminergic cardiomyocyte population expressing Dbh that contributes to the murine cardiac conduction system through transcriptomic, genetic, and optogenetic analyses.

Cuproptosis is correlated with clinical status, tumor immune microenvironment and immunotherapy in colorectal cancer: a multi-omic analysis

This study identifies 16 cuproptosis-related molecules and develops a scoring system to predict prognosis, tumor immune microenvironment, and immunotherapy response in colorectal cancer.

Control of Motor Landing and Processivity by the CAP-Gly Domain in the KIF13B Tail

This study investigates how the CAP-Gly domain in KIF13B affects its interaction with tyrosinated microtubules, showing it influences initial binding and processive motility.

Enhanced Single RNA Imaging Reveals Dynamic Gene Expression in Live Animals

This paper introduces a method to image endogenous mRNAs in live animals with high temporal resolution and applies it to study gene expression dynamics in C. elegans epidermis.

Generation of cardiomyocytes from human induced pluripotent stem cells resembling atrial cells with ability to respond to adrenoceptor agonists

This paper describes a method to generate human atrial-like cardiomyocytes from induced pluripotent stem cells that respond to adrenoceptor agonists.