Author

M. Legut

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Approach:experimental assay· 2 Research Focus:perturbation response prediction· 2 Approach:genetic perturbation / CRISPR· 1 Organism:human· 1

Frequent Co-Authors

H.-H. Wessels· 2 shared N. E. Sanjana· 2 shared A. Kadina· 1 shared A. Mendez-Mancilla· 1 shared D. McKenzie· 1 shared G. B. Robb· 1 shared J. Walker· 1 shared K. Holden· 1 shared

Activity by Year

2020-2021 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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2 shared papersBioengineering

H.-H. Wessels

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2 papers

Recent·Most cited

Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells

A. Mendez-Mancilla, H.-H. Wessels, M. Legut, A. Kadina, M. Mabuchi, J. Walker, G. B. Robb, K. Holden, N. E. Sanjana

The paper identifies chemically modified crRNAs that enhance CRISPR-Cas13 knockdown efficiency and half-life in human cells.

bioRxiv · May 12, 2021Bioengineering

High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation

M. Legut, Z. Daniloski, X. Xue, D. McKenzie, X. Guo, H.-H. Wessels, N. E. Sanjana

The paper compares PAM-flexible Cas9 variants (xCas9 and Cas9-NG) and wild-type Cas9 across gene knock-out, activation, and suppression using a high-throughput screen, finding that PAM flexibility reduces targeting efficiency and that Cas9-NG outperforms xCas9.

bioRxiv · Jan 22, 2020Bioengineering