N. K. Devaraj

Semisynthesis of functional transmembrane proteins in GUVs

This study semisynthesizes functional transmembrane proteins in GUVs using split intein-mediated ligation, demonstrating functional retention of PD-1 in reconstituted membranes.

RNA-CLAMP Enables Photo-activated Control of CRISPR-Cas9 Gene Editing by Site-specific Intramolecular Cross-linking of the sgRNA

RNA-CLAMP enables photo-activated control of CRISPR-Cas9 gene editing by clamping and photo-cleaving sgRNA stem loops to regulate activity.

Light-activated tetrazines enable live-cell spatiotemporal control of bioorthogonal reactions

This paper introduces light-activated tetrazines to enable precise spatiotemporal control of bioorthogonal reactions in live cells.

A Small Molecule Fluorogenic Probe for the Detection of Sphingosine in Living Cells

This paper develops a fluorescent probe to detect sphingosine in living cells and demonstrates its use in detecting sphingosine accumulation in NPC1 patient cells.

Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein Interactions

This paper introduces an enzymatic RNA biotinylation method to identify RNA-protein interactions and enrich RNA in mammalian cells, demonstrating its utility in identifying binding partners of 7SK snRNA and HOTAIR.

Multiplexed photo-activation of mRNA with single-cell resolution

This paper demonstrates sequential optical activation of two mRNAs in the same cell using photo-cages, enabling precise control of translation with single-cell resolution.

Enzyme-free synthesis of natural phospholipids in water

This paper demonstrates an enzyme-free method to synthesize natural phospholipids in water, forming cell-like membranes and establishing proton gradients.

Laccase-mediated catalyzed fluorescent reporter deposition for live cell imaging

This paper introduces a laccase-based CARD method for live-cell imaging that avoids hydrogen peroxide, demonstrating its feasibility with fluorescent labeling of proteins.