BiophysicsbioRxiv
NMR measurements of transient low-populated tautomeric and anionic Watson-Crick-like G·T/U in RNA:DNA hybrids: Implications for the fidelity of transcription and CRISPR/Cas9 gene editing
O. Szekely, A. K. Rangadurai, S. Gu…·H. Al-Hashimi
This study uses NMR to show that G*T/U mismatches in RNA:DNA hybrids can form Watson-Crick-like conformations, potentially contributing to transcription errors and CRISPR/Cas9 off-target effects.
DOI 10.1093/nar/gkae027