S. Gu

The transcription factor Zeb2 drives differentiation of age-associated B cells

The paper identifies Zeb2 as a critical transcription factor driving age-associated B-cell differentiation and autoimmunity in humans and mice.

Direct Measurement of 8OG syn-anti Flips in Mutagenic 8OG·A and Long-Range Damage-Dependent Hoogsteen Breathing Dynamics Using 1H CEST NMR

This paper measures 8OG syn-anti flips and Hoogsteen breathing dynamics in damaged DNA using 1H CEST NMR, revealing their impact on DNA conformation and repair.

Host signature as major driver of root and rhizosphere core microbiomes that differently affect plant functional traits

The paper identifies plant traits as the main driver of core microbiomes in different root compartments and shows their distinct impacts on plant metal accumulation and growth.

Molecular determinants of antagonist interactions with chemokine receptors CCR2 and CCR5

This study identifies molecular determinants of CCR2 and CCR5 antagonist interactions using docking and AI screening, revealing structural principles and selectivity mechanisms.

Siderophore synthetase-receptor gene coevolution reveals habitat- and pathogen-specific bacterial iron interaction networks

The paper develops a co-evolution algorithm to predict bacterial iron interaction networks based on siderophore synthetase-receptor gene pairs across Pseudomonas genomes, revealing habitat- and pathogen-specific interactions.

Ligand-Dependent Mechanisms of C-C Chemokine Receptor 5 (CCR5) Trafficking Revealed by APEX2 Proximity Labeling Proteomics

This study uses APEX2 proximity labeling and mass spectrometry to uncover ligand-dependent CCR5 trafficking mechanisms, revealing distinct internalization and degradation pathways.

SIDERITE: Unveiling Hidden Siderophore Diversity in the Chemical Space Through Digital Exploration

SIDERITE identifies new iron-binding molecules by analyzing chemical structures and confirming their iron-binding ability through experimental validation.

NMR measurements of transient low-populated tautomeric and anionic Watson-Crick-like G·T/U in RNA:DNA hybrids: Implications for the fidelity of transcription and CRISPR/Cas9 gene editing

This study uses NMR to show that G*T/U mismatches in RNA:DNA hybrids can form Watson-Crick-like conformations, potentially contributing to transcription errors and CRISPR/Cas9 off-target effects.

Recombinant SARS-CoV-2 lacking initiating and internal methionine codons within ORF10 is attenuated in vivo

This study shows that a recombinant SARS-CoV-2 virus lacking ORF10 start and internal methionine codons is attenuated in hamsters, with reduced lower respiratory tract viral titre and increased reversion to wild-type.

Rat microbial biogeography and age-dependent lactic acid bacteria in healthy lungs

This study defines the microbial biogeography of healthy Fischer 344 rats across their lifespan and identifies age-dependent changes in lactic acid bacteria in the lungs.

Curing GFP-itis in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students

This paper describes a three-day educational program teaching high school students genome editing through a base editing experiment in E. coli, including background lectures and ethics discussions.

Enzyme encapsulation peptides bind to the groove between tessellating subunits of the bacterial microcompartment shell

The paper identifies a hydrophobic groove between bacterial microcompartment shell proteins as the binding site for encapsulation peptides.

Siderophore-mediated iron partition promotes dynamical coexistence between cooperators and cheaters

The paper shows that siderophore-mediated iron partition enables stable coexistence between cheaters and partial cooperators in microbial communities.

Connexin 43 Hemichannels Regulate Mitochondrial ATP Generation, Mobilization, and Mitochondrial Homeostasis against Oxidative Stress

This study shows that mitochondrial Cx43 hemichannels regulate mitochondrial ATP production and protect osteocytes from oxidative stress by facilitating ion and ATP transfer and interacting with ATP synthase.

Large library docking for novel SARS-CoV-2 main protease non-covalent and covalent inhibitors

The paper identifies novel non-covalent and covalent inhibitors of SARS-CoV-2 main protease through virtual docking and experimental validation.

Label-free Cell Tracking Enables Collective Motion Phenotyping in Epithelial Monolayers

The paper introduces an AI-based pipeline to track cell nuclei in phase contrast images and uses it to analyze collective motion in epithelial monolayers, finding that D2min correlates with metastatic potential.

Data-driven discovery of targets for bipotent anticancer drugs identifies Estrogen Related Receptor Alpha

BipotentR identifies immune-metabolic regulators for bipotent anticancer drugs, demonstrating ESRRA inhibition's effects on tumor metabolism and immune mechanisms.

A molecular switch between mammalian MLL complexes dictates response to Menin-MLL inhibition

The paper identifies a molecular switch between MLL1-Menin and MLL3/4-UTX complexes that determines response to Menin-MLL inhibitors in leukemia.

Scaffolding protein GspB/OutB facilitates assembly of the Dickeya dadantii type 2 secretion system by anchoring the outer membrane secretin pore to the inner membrane and to the peptidoglycan cell wall.

This paper shows that GspB scaffolds the T2SS secretin pore in Dickeya dadantii by anchoring it to the inner membrane and peptidoglycan, and identifies its conserved periplasmic domain and interactions with GspD.

High-throughput phenotyping analysis of maize at the seedling stage using end-to-end segmentation network

This paper introduces PlantU-net, an end-to-end segmentation network for maize seedling phenotyping from top-view images, achieving high accuracy with minimal training data.

Tumor-Immune Partitioning and Clustering (TIPC) algorithm reveals distinct signatures of tumor-immune cell interactions within the tumor microenvironment

The paper introduces TIPC, a computational algorithm that identifies tumor-immune spatial patterns in colorectal cancer, linking them to patient survival and tumor features.

Counteracting Genome Instability by p53-dependent Mintosis

The paper identifies a p53-dependent process called mintosis that eliminates aneuploid mitotic progenies through entosis to counteract genome instability.

Genome-wide CRISPR screens identify essential cell growth mediators in BRAF-inhibitor resistant melanoma

The paper identifies key regulators of BRAF inhibitor resistance in melanoma using CRISPR screens and integrates multi-omics data to reveal CDK6-dependent mechanisms.

Phosgene Inhalation Causes Hemolysis and Acute Lung Injury

This study investigates the effects of phosgene exposure on mice, showing dose-dependent lung injury and hemolysis, with free heme and membrane breakdown products as potential biomarkers.

SHP2 Is Required for BCR-ABL1-Induced Hematologic Neoplasms

This study shows SHP2 is essential for BCR-ABL1-induced hematologic cancers, affecting proliferation and signaling pathways in pre-B cells.