BiochemistrybioRxiv
PAM binding ensures orientational integration during Cas4-Cas1-Cas2 mediated CRISPR adaptation
Y. Dhingra, S. K. Suresh, P. Juneja, D. G. Sashital
This study determines the cryo-EM structures of the Cas4-Cas1-Cas2 complex, revealing how PAM binding ensures correct spacer orientation during CRISPR adaptation.
DOI 10.1016/j.molcel.2022.09.030