Author
Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.
5 papers
Recent·Most cited
This study identifies a type I-G CRISPR-Cas system where Cas4/1 fusion protein performs dual nuclease activities for prespacer maturation and directional integration.
Y. Dhingra, S. K. Suresh, P. Juneja, D. G. Sashital
This study determines the cryo-EM structures of the Cas4-Cas1-Cas2 complex, revealing how PAM binding ensures correct spacer orientation during CRISPR adaptation.
M. A. Schelling, G. T. Nguyen, D. G. Sashital
This study shows that Cas12a mismatch tolerance and pre-existing target mismatches influence phage evolution, with liquid media leading to more phage escape despite in vitro cleavage activity.
K. Murugan, A. S. Seetharam, A. J. Severin, D. G. Sashital
This study compares cleavage activities and specificities of natural and engineered Cas9 variants using a high-throughput in vitro assay, revealing differences in off-target nicking and DSB formation.
K. Murugan, A. Seetharam, A. J. Severin, D. G. Sashital
This study investigates Cas12a cleavage specificity, revealing pervasive off-target nicking and double-stranded DNA degradation, which may impact its use in genome editing.