Author

T. A. Leonard

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5

Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Research Focus:protein folding & dynamics· 4 Approach:biophysical modeling· 2 Research Focus:cell signaling & trafficking· 2 Assay:protein structure· 2 Organism:C. elegans· 1 Research Focus:chromatin & epigenetic regulation· 1 Organism:human· 1

Frequent Co-Authors

J. E. Burke· 4 shared L. Truebestein· 3 shared K. D. Fleming· 2 shared A. Hausser· 1 shared A. L. Shaw· 1 shared A. Levina· 1 shared A.-C. Gavin· 1 shared C. Gehin· 1 shared

Activity by Year

2021-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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4 shared papersBiochemistry

J. E. Burke

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Author profile →

5 papers

Recent·Most cited

BiochemistrybioRxivPreprintJul 14, 2022

ATP-competitive and allosteric inhibitors induce differential conformational changes at the auto-inhibitory interface of Akt

A. L. Shaw, M. A. Parson, L. Truebestein…·J. E. Burke

This study investigates how ATP-competitive and allosteric inhibitors induce different conformational changes in Akt's auto-inhibitory interface across various phosphorylation states.

DOI 10.1101/2022.07.14.499806

protein structureexperimental assay

𝕏 PostBluesky

BiochemistrybioRxivMay 5, 2022

PKD autoinhibition in trans regulates activation loop autophosphorylation in cis

R. Reinhardt, K. Hirzel, G. Link…·T. A. Leonard

This study shows PKD is regulated by trans-autoinhibition followed by cis autophosphorylation, switching the kinase on by preventing re-autoinhibition.

DOI 10.1073/pnas.2212909120

humanprotein structure

𝕏 PostBluesky

BiochemistrybioRxivMar 11, 2022

Structure and regulation of the Myotonic dystrophy kinase-Related Cdc42-binding Kinase

L. Truebestein, E. Waltenberger, C. Gehin…·T. A. Leonard

The paper presents the structure and membrane-binding properties of C. elegans MRCK1, revealing its homodimeric conformation and interactions with phosphoinositides and Cdc42.

DOI 10.1016/j.str.2023.02.002

C. elegansexperimental assay

𝕏 PostBluesky

BiochemistrybioRxivOct 8, 2021

Activation of the essential kinase PDK1 by phosphoinositide-driven autophosphorylation

A. Levina, K. D. Fleming, J. E. Burke, T. A. Leonard

This study reveals how PDK1 is activated by phosphoinositides through autophosphorylation via a dimer mechanism and PH domain regulation.

DOI 10.1038/s41467-022-29368-4

proteomicsexperimental assay

𝕏 PostBluesky

BiochemistrybioRxivApr 13, 2021

In vitro reconstitution of Sgk3 activation by phosphatidylinositol-3-phosphate

D. Pokorny, L. Truebestein, K. D. Fleming…·T. A. Leonard

This study shows that Sgk3 is activated by PI3P through conformational changes and reconstitutes this activation in vitro using Vps34-mediated PI3P synthesis on liposomes.

DOI 10.1016/j.jbc.2021.100919

experimental assay

𝕏 PostBluesky