J. E. Burke

This study identifies spartin as a lipid transfer protein essential for lipid droplet turnover via lipophagy, revealing a new lipid transport module in its senescence domain.

This study investigates ESAT-6's self-association under acidic conditions and shows an ESAT-6-specific nanobody inhibits M. tuberculosis growth in macrophages.

This study identifies the helical domain of p110γ as an allosteric regulator of PI3Kγ activity, showing distinct roles in p110γ-p84 and p110γ-p101 complexes through phosphorylation or nanobody binding.

The paper investigates how lipid composition, specifically phosphatidic acid and diacylglycerol, affects PLIN3 recruitment to membranes and LDs, revealing structural changes in the PAT domain.

This study identifies molecular differences between p110γ-p84 and p110γ-p101 complexes that explain their differential activation by GPCRs and Ras.

This study investigates how ATP-competitive and allosteric inhibitors induce different conformational changes in Akt's auto-inhibitory interface across various phosphorylation states.

This study shows PKD is regulated by trans-autoinhibition followed by cis autophosphorylation, switching the kinase on by preventing re-autoinhibition.

This study identifies unique regulatory mechanisms by which oncogenic PIK3CA mutations enhance PI3K membrane recruitment and activation through structural reorientations.

This study shows that PLC-gamma1 becomes active upon binding to FGFR1 kinase domain and membrane-bound PIP2, with an oncogenic mutation enhancing this activation.

This study reveals how PDK1 is activated by phosphoinositides through autophosphorylation via a dimer mechanism and PH domain regulation.

This study identifies palmitoylation as a mechanism that localizes CNA{beta}1 to the plasma membrane and Golgi, enabling its interaction with the PI4KA complex and regulating PI4P production.

The paper reports the cryo-EM structure of the p110γ-p101 complex, revealing how GPCR activation is mediated through interactions with Gβγ and identifying potential therapeutic targets.

This study investigates the GEF activity of TRAPPIII and TRAPPII complexes toward Rab GTPases using biochemical and structural methods, revealing Rab specificity and membrane interaction mechanisms.

This paper uses HDX-MS to characterize nanobodies targeting PI3K{gamma} complexes, identifying epitopes and functional effects on lipid kinase activity and GPCR activation.

This study shows that Sgk3 is activated by PI3P through conformational changes and reconstitutes this activation in vitro using Vps34-mediated PI3P synthesis on liposomes.

The paper identifies the M-Lip domain as a novel dimeric protein fold that binds membranes and is essential for lipin function.

The paper determines the crystal structure of an archaeal CNNM protein bound to Mg2+-ATP, revealing its transmembrane fold and suggesting it functions as a cation transporter.

This study investigates how mutations in PI3Kγ affect its regulatory dynamics and identifies a strategy for developing selective inhibitors.

This study investigates how Gβγ and Gαq activate PLCβ by inducing structural changes in its C-terminal domain, leading to increased hydrolase activity.

This study investigates how mutations in PIK3R1 affect class IA PI3K regulation, finding that oncogenic mutations hyper-activate p110 while developmental mutations reduce RTK sensitivity.

This study characterizes the c10orf76-PI4KB complex, showing its role in maintaining Golgi PI4P levels and enabling enterovirus replication.