Author

K. D. Fleming

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 9

Signature Assays, Organisms & Methods

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Approach:experimental assay· 8 Research Focus:cell signaling & trafficking· 4 Research Focus:protein folding & dynamics· 4 Approach:biophysical modeling· 2 Research Focus:drug response / drug discovery· 2 Assay:protein structure· 2 Assay:proteomics· 2 Assay:structural biology· 2

Frequent Co-Authors

J. E. Burke· 9 shared C. K. Yip· 4 shared M. K. Rathinaswamy· 4 shared U. Dalwadi· 4 shared M. L. Jenkins· 3 shared N. J. Harris· 3 shared E. Pardon· 2 shared J. Steyaert· 2 shared

Activity by Year

2020-2022 · peak 6/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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9 shared papersBiochemistry

J. E. Burke

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Author profile →

9 papers

Recent·Most cited

Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulation

Y.-M. Choi, D. Ajjaji, K. D. Fleming, P. P. Borbat, M. L. Jenkins, B. E. Moeller, S. Fernando, S. R. Bhatia, J. H. Freed, J. E. Burke, A. R. Thiam, M. V. Airola

The paper investigates how lipid composition, specifically phosphatidic acid and diacylglycerol, affects PLIN3 recruitment to membranes and LDs, revealing structural changes in the PAT domain.

bioRxiv · Nov 17, 2022Biochemistry

Molecular basis for differential activation of p101 and p84 complexes of PI3Kγ by Ras and GPCRs

M. K. Rathinaswamy, M. L. Jenkins, X. Zhang, J. T. Stariha, H. Ranga-Prasad, U. Dalwadi, K. D. Fleming, C. K. Yip, R. L. Williams, J. E. Burke

This study identifies molecular differences between p110γ-p84 and p110γ-p101 complexes that explain their differential activation by GPCRs and Ras.

bioRxiv · Jul 29, 2022Biochemistry

Activation of the essential kinase PDK1 by phosphoinositide-driven autophosphorylation

A. Levina, K. D. Fleming, J. E. Burke, T. A. Leonard

This study reveals how PDK1 is activated by phosphoinositides through autophosphorylation via a dimer mechanism and PH domain regulation.

bioRxiv · Oct 8, 2021Biochemistry

Structure of the phosphoinositide 3-kinase p110γ-p101 complex reveals molecular mechanism of GPCR activation

M. K. Rathinaswamy, U. Dalwadi, K. D. Fleming, C. Adams, J. T. Stariha, E. Pardon, M. Baek, O. Vadas, F. DiMaio, J. Steyaert, S. D. Hansen, C. K. Yip, J. E. Burke

The paper reports the cryo-EM structure of the p110γ-p101 complex, revealing how GPCR activation is mediated through interactions with Gβγ and identifying potential therapeutic targets.

bioRxiv · Jun 1, 2021Biochemistry

Biochemical insight into novel Rab-GEF activity of the mammalian TRAPPIII complex

N. J. Harris, M. L. Jenkins, U. Dalwadi, K. D. Fleming, S.-E. Nam, M. A. Parson, C. K. Yip, J. E. Burke

This study investigates the GEF activity of TRAPPIII and TRAPPII complexes toward Rab GTPases using biochemical and structural methods, revealing Rab specificity and membrane interaction mechanisms.

bioRxiv · Jun 1, 2021Biochemistry

HDX-MS optimized approach to characterize nanobodies as tools for biochemical and structural studies of class IB phosphoinositide 3-kinases

M. K. Rathinaswamy, K. D. Fleming, U. Dalwadi, E. Pardon, N. J. Harris, C. K. Yip, J. Steyaert, J. E. Burke

This paper uses HDX-MS to characterize nanobodies targeting PI3K{gamma} complexes, identifying epitopes and functional effects on lipid kinase activity and GPCR activation.

bioRxiv · Jun 1, 2021Biochemistry

In vitro reconstitution of Sgk3 activation by phosphatidylinositol-3-phosphate

D. Pokorny, L. Truebestein, K. D. Fleming, J. E. Burke, T. A. Leonard

This study shows that Sgk3 is activated by PI3P through conformational changes and reconstitutes this activation in vitro using Vps34-mediated PI3P synthesis on liposomes.

bioRxiv · Apr 13, 2021Biochemistry

The middle lipin (M-Lip) domain is a new dimeric protein fold that binds membranes

W. Gu, S. Gao, H. Wang, K. D. Fleming, R. M. Hoffmann, J. W. Yang, N. M. Patel, Y. M. Choi, J. E. Burke, K. Reue, M. V. Airola

The paper identifies the M-Lip domain as a novel dimeric protein fold that binds membranes and is essential for lipin function.

bioRxiv · Mar 19, 2021Biochemistry

Disease related mutations in PI3Kγ disrupt regulatory C-terminal dynamics and reveals a path to selective inhibitors

M. K. Rathinaswamy, Z. Gaieb, K. D. Fleming, C. Borsari, N. J. Harris, B. J. Moeller, M. P. Wymann, R. E. Amaro, J. E. Burke

This study investigates how mutations in PI3Kγ affect its regulatory dynamics and identifies a strategy for developing selective inhibitors.

bioRxiv · Dec 1, 2020Biochemistry