T. Surrey

The minus end depolymerase KIF2A drives flux-like treadmilling of γTuRC-uncapped microtubules

This study shows KIF2A drives treadmilling of γTuRC-uncapped microtubules by depolymerizing minus ends while allowing nucleation from γTuRC.

Microtubule binding of the human HAUS complex is directly controlled by importins and Ran-GTP

The paper shows that Ran-GTP relieves importin-mediated inhibition of HAUS complex microtubule binding, directly regulating microtubule nucleation during mitosis.

Effects of microtubule length and crowding on active microtubule network organization

This study uses simulations and experiments to show that microtubule length and crowding influence active network organization, leading to different dynamic architectures.

Crosslinker design determines microtubule network organization by opposing motors

This study investigates how different crosslinker designs influence microtubule network organization by opposing motors, revealing that asymmetric crosslinkers can support both contractile and extensile behaviors.

Structural transitions in the GTP cap visualized by cryo-EM of catalytically inactive microtubules

The paper uses cryo-EM to visualize the structure of GTP-bound microtubules, revealing an expanded lattice and closed seam that stabilize the GTP cap.

Quantifying the monomer-dimer equilibrium of tubulin with mass photometry

This study uses mass photometry to quantify the monomer-dimer equilibrium of tubulin, clarifying its energetics and kinetics.

Gradual Compaction of the Central Spindle Decreases its Dynamicity as Revealed in PRC1 and EB1 Gene-Edited Human Cells

The paper quantifies dynamic changes in the central spindle during mitosis in human cells using gene-edited fluorescent markers and finds it becomes more stable as it compacts.

Microtubule Nucleation by Single Human γTuRC in a Partly Open Asymmetric Conformation

This study characterizes the nucleation properties of human γTuRC using TIRF microscopy and cryo-EM, revealing an asymmetric conformation that explains its kinetic inhibition of microtubule nucleation.

The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability

This study shows that GTP hydrolysis speed affects GTP cap size and microtubule stability by influencing EB protein binding and regulatory hub formation.

Combinatorial Regulation Of The Balance Between Dynein Microtubule End Accumulation And Initiation Of Directed Motility

The paper investigates how dynein activity is regulated by different regulators, revealing distinct pools of dynein with opposing preferences for binding regulators and different motility behaviors.