Kate E. Galloway

This paper designs single-cargo synthetic gene circuits that generate two balanced cellular states without relying on copy-number variation.

This paper demonstrates that self-amplifying RNA (saRNA) enables durable, integration-free transgene expression in hiPSCs, supporting cell-fate programming and functional reporter analysis.

This paper introduces a miRNA feedback circuit to control synthetic receptor activity, showing tunable and autonomous regulation of synNotch receptor expression.

This study develops high-titer lentiviral vectors for RNA-based gene circuits by optimizing gene syntax and genetic parts to enhance vector production and expression.

STRAIGHT-IN Dual enables efficient, single-copy integration of two DNA payloads into hiPSCs, improving genetic engineering for synthetic biology applications.

The paper shows that gene syntax influences supercoiling-mediated transcriptional feedback, affecting adjacent gene expression in human cells.

This paper introduces DASIT, a protein-based circuit for antigen-specific cell selection, demonstrating its utility in genome engineering, CRISPR screening, and cell therapy manufacturing.

DIAL is a framework for designing synthetic promoters that generate heritable, tunable gene expression setpoints across cell types.

This study shows that optimal MAPK signaling levels enhance direct conversion of fibroblasts to motor neurons, while excessive levels induce senescence and reduce conversion rates.

This paper introduces RE array plasmids and the CTRL method to control gene expression by sequestering transcription factors in mammalian cells.

The paper designs and characterizes ComMAND, a microRNA-based gene circuit that precisely controls transgene expression in diverse primary cells.

The paper measures mRNA and protein levels in single cells for synthetic promoters, finding that promoter strength affects both transcription and translation, and introduces a framework for comparing gene regulation.

The paper identifies a quiescent-like state with reduced nuclear size and transcriptional activity that enables efficient TF-mediated conversion of fibroblasts to neurons.

This study identifies proliferation history and transcription factor levels as key drivers of direct conversion of fibroblasts to motor neurons using a synthetic biology approach.

This study models how supercoiling-mediated feedback couples gene expression in synthetic and native systems, showing it influences expression profiles, variance, and regulatory interactions.