Stephen H. McLaughlin

GuideFlip co-designs protein structure and sequence for flexible targets, improving in silico success rates and experimentally validating binders to disordered proteins.

This paper identifies DNA aptamers that bind the truncated form of HasA, a hemophore in Pseudomonas aeruginosa, under acidic conditions, showing potential as antimicrobial agents.

This paper describes a method to purify and characterize soluble domains of bacterial membrane receptors, demonstrating its effectiveness with the HasR receptor from Pseudomonas aeruginosa.

This study uses cryo-EM to reveal structural mechanisms of KMN complex assembly and phospho-regulation in budding yeast kinetochores.

The paper determines cryo-EM structures of the CCAN complex bound to different DNA sequences to understand its architecture on centromeric α-satellite CENP-A nucleosome arrays.

The paper identifies a kinetic ruler mechanism where Nab2 protein concentration determines mRNA poly(A) tail length through competition between polyadenylation and RNA-binding.

This study determines the cryo-EM structure of the MmpS5L5 efflux pump in Mycobacterium tuberculosis and identifies mutations that enhance bedaquiline efflux, suggesting new resistance mechanisms.

This study uses cryo-EM to determine high-resolution structures of apo-APC/C and APC/C CDH1:EMI1, revealing new structural features and regulatory mechanisms.

This study reveals how substrate-induced clustering activates TRIM21's ubiquitination activity for antiviral responses and protein degradation, introducing optogenetically controllable TRIM21-nanobody chimeras.

This study reveals that bactofilins form non-polar filaments that bind directly to membranes, with conserved N-terminal tails mediating membrane interaction.