Shasha Shi

This study shows that U1C and Helix H of U1 snRNA are essential for branaplam's effect on weak 5 splice site recognition, while risdiplam requires additional factors for activity.

The paper investigates the interaction between U1 snRNP and RNA polymerase II in yeast, finding that Prp40, not U1-70K, mediates this interaction, contrasting with the human system.

The paper determines cryo-EM structures of PP2A-B55 and Eya3 interaction, showing how a peptide inhibitor disrupts this interaction and affects Myc stability in TNBC.

The paper humanizes yeast U1 snRNP components to study 5 ss recognition, finding that U1C ZnF substitution causes splicing defects and mitochondrial dysfunction.