Mark J. Skehel

This study identifies factors governing cysteine detection in chemoproteomics using machine learning and statistical filtering, showing that peptide properties and abundance are key determinants.

This study shows TRF2 mediates force-resistant tethering of telomeric DNA repeats, which may be a step in T-loop formation.

This paper introduces FlyMOE, a chemical-genetic platform for targeted glycoproteome analysis in Drosophila, using mass spectrometry to study glycosylation in vivo.

This study develops an in vitro system to study SERA6 maturation and validates inhibitors that block malaria parasite egress.

Yeast m6A reader Pho92 uses one helical hydrophobic interface in its disordered region to engage Caf40; a single L35A substitution halts m6A-transcript decay in meiosis.

This study develops BacPROTACs, a new class of antibacterial agents targeting M. tuberculosis, showing improved activity over traditional inhibitors.

The paper identifies a covalent fragment inhibitor of TYMS that selectively kills ATRX-deficient cells through replication stress.

This study establishes isogenic NPC1 and NPC2 models to reveal that HKDC1 regulates mitochondrial-lysosome contact in NPC1-deficient cells.

This study identifies CDKL5 as a kinase that phosphorylates nELAVL proteins, enhancing mRNA binding and protein synthesis, and shows its role in visual cortex development.

The paper shows that a truncated ASH2L isoform, generated via a lineage-specific retrotransposon, primes developmental gene promoters during pluripotency and is required for later developmental transitions.

This study performs phosphoproteomic analysis of TSC brain lesions, finding strong mTORC1 activation and widespread mRNA splicing changes in SEGAs but not tubers.

The paper investigates how chaperones like Hsp70, Hsp90, and TRiC bind to nascent p53 during translation, showing TRiC's specificity and how mutations affect this process.

This study investigates the biogenesis of the SARS-CoV-2 M protein, revealing key steps in its insertion, folding, and oligomerization.

This study investigates how GroEL/ES interacts with nascent proteins on ribosomes, showing that GroEL binds and destabilizes substrates, while GroES facilitates partial encapsulation and refolding.

The paper investigates how the human ribosome modulates multidomain protein folding by delaying domain docking, contrasting with bacterial ribosomes.

The paper characterises LAMP1- and LAMP2A-positive organelles in neurons, finding they have distinct transport dynamics and protein compositions.

The paper investigates PADI6's role in oocyte cytoplasmic lattices using single-cell proteomics and transcriptomics, revealing its critical scaffolding function and connections to other oocyte compartments.

This paper develops a label-free chemoproteomics platform to profile cysteine-reactive fragments, identifying over 400 ligand-protein interactions in two cell lines.

This study shows that PP7 is essential for early ring-stage development in Plasmodium falciparum but not for erythrocyte invasion.

The paper compares protein dynamics in mouse and human neural progenitors, finding that human NPs have longer protein half-lives and lower proteasomal activity, which influences developmental tempo.

This paper identifies the V1H subunit of V-ATPase as a regulator of ATG16L1 recruitment in response to pH gradient disruption, linking V-ATPase function to autophagy activation.

This study shows that PKG regulation in malaria parasites involves cGMP saturation and activation loop phosphorylation, with phosphorylation being essential for in vivo function.

The paper investigates how chaperones coordinate during cotranslational protein folding in bacteria, showing they create a protected space for maturation without destabilizing intermediates.

The paper shows how Rim11 integrates signals from PKA, TORC1, and Ime1 to regulate Ume6 phosphorylation and drive meiotic gene expression in yeast.

The paper identifies three novel components of the yeast m6A methyltransferase complex and reveals their distinct m6A-dependent and independent roles in meiosis and mating.

The paper introduces BOCTAG, a method to label glycoproteins in specific cell types within mixed cultures, enabling analysis of cell-specific glycosylation.

This study shows that dual loss of NDR1/2 kinases in neurons causes neurodegeneration, disrupts endocytosis, and impairs autophagy by affecting membrane trafficking and protein homeostasis.

The paper shows Mpe1 interacts with the polyadenylation signal in pre-mRNA to activate CPF and control polyadenylation in yeast.