Lan Huang

This paper introduces xGL-APMS to profile endogenous galectin-3 interactions at the cell surface, identifying 125 interactors on Jurkat cells and 69 on mouse thymocytes.

The paper evaluates DSSO crosslinking strategies using Protein Prospector and Touchstone, finding that DSSO's advantage comes from backbone fragmentation, not diagnostic ions, and that MS3 is better for proteome-scale samples.

This paper shows that an orthosteric inhibitor of the COP9 signalosome achieves high potency through a molecular glue mechanism that enhances substrate-dependent binding.

This paper introduces a novel trioxane-based cross-linker, TSTO, for XL-MS that enables fast and unambiguous identification of multimeric protein interactions, revealing new structural details of protein complexes.

The paper identifies NEMF-mediated CAT-tailing as a mechanism that directs substrates through distinct degradation pathways during translocation-associated quality control.

PL-ExM combines proximity labeling and expansion microscopy to visualize 3D interactome structures and assess PL technique performance.

The paper investigates how ZFAND5 activates the 26S proteasome by inducing conformational changes that enhance substrate degradation.

This study discovers that native MCC from Leishmania tarentolae forms filaments, determines its structure, and proposes a new model for its catalytic mechanism.

This study investigates the role of the co-receptor's ligand-binding domain in the assembly and trafficking of olfactory Ionotropic Receptors, revealing its importance in endoplasmic reticulum exit.