Jonathan T. Vu

This study shows that PEX1 deficiencies disrupt HIF1α proteostasis via autophagy, leading to increased HIF1 levels and impaired recovery under hypoxia-reoxygenation.

POCKET-seq maps dCas9 binding genome-wide to distinguish true and false-positive interactions in CRISPR interference screens.

The paper identifies RNF146 and tankyrase as regulators of peroxisome function and links them to Wnt signaling through their role in PARsylation and beta-catenin degradation.

The paper shows that interstrand crosslinked DNA templates (xHDRTs) significantly boost gene editing efficiency in human cells by up to five-fold, independent of template topology or cell type.

This study uses CRISPR base editing to identify novel mutations in the HBG promoter that increase fetal hemoglobin expression without harmful effects.

This paper introduces DISCOVER-Seq, a method to detect CRISPR off-targets in vivo with single-base resolution, applicable to multiple Cas nucleases.

This study uses CRISPR-Cas9 to test a putative fetal globin repressor region, finding it does not autonomously silence gamma-globin, suggesting it is not a therapeutic target for beta-hemoglobinopathies.

Cas9-mediated correction of the sickle mutation in human hematopoietic stem cells achieves curative levels of editing.

This study shows that DNA double-strand breaks cause ribosome remodeling and translational shutdown, independent of p53 and preceding transcriptional changes.