Author

Tomasz Śpiewla

University of WarsawORCID 0000-0002-6542-3966Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:drug response / drug discovery· 2 Assay:proteomics· 2 Assay:bulk RNA-seq· 1 Approach:deep learning· 1 Research Focus:host-pathogen & immune response· 1 Assay:metagenomics· 1 Organism:mouse· 1

Frequent Co-Authors

Jacek JemielityiD· 4 shared Joanna KowalskaiD· 4 shared Dominika NowisiD· 3 shared Jakub GołąbiD· 3 shared Marcin WarmińskiiD· 3 shared Adam MamotiD· 2 shared Andrzej DziembowskiiD· 2 shared Karol CzubakiD· 2 shared

Activity by Year

2024-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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4 shared papersBiochemistry

Jacek JemielityORCID

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4 papers

Recent·Most cited

Molecular BiologyNucleic Acids ResearchPeer-reviewedDec 2, 2025

PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA

Tomasz Śpiewla, Karol Czubak, Zofia Pilch…·Joanna Kowalska

The paper develops segmented polyA tail designs to improve DNA stability and mRNA translatability, finding that longer segmented tails significantly enhance protein production.

10 citations

experimental assay

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BiochemistryNature CommunicationsPeer-reviewedJul 12, 2025

Chemical circularization of in vitro transcribed RNA opens new avenues for circular mRNA design

Małgorzata Wąsińska-Kałwa, Adam Mamot, Karol Czubak…·Jacek Jemielity

This paper introduces a chemical method to circularize in vitro transcribed RNA, producing chemically circularized RNAs (chem-circRNAs) with high efficiency and compatibility with modifications.

24 citationstop 2%

bulk RNA-seqexperimental assay

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BiochemistryRNAPeer-reviewedDec 6, 2024

An MST-based assay reveals new binding preferences of IFIT1 for canonically and non-canonically capped RNAs

Tomasz Śpiewla, Katarzyna Grab, Anaïs Depaix…·Joanna Kowalska

This study develops an MST-based assay to assess IFIT1 binding to various capped RNAs, revealing new preferences for canonical and non-canonical RNA caps.

3 citations

proteomicsexperimental assay

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BiochemistryJournal of the American Chemical SocietyPeer-reviewedMar 5, 2024

Trinucleotide mRNA cap analog N6-benzylated at the site of posttranscriptional m6Am mark facilitates mRNA purification and confers superior translational properties in vitro and in vivo

Marcin Warmiński, Edyta Trepkowska, Mirosław Śmietański…·Joanna Kowalska

This paper introduces a modified mRNA cap analog, AvantCap, which improves mRNA purification and translational efficiency in vitro and in vivo.

44 citationstop 3%

mousemetagenomics

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