Tyler L. Dangerfield

This study uses pre-steady state kinetics and global data fitting to analyze CRL-dependent ubiquitylation, revealing kinetic parameters and catalytic efficiencies across different CRL systems and substrate contexts.

The paper characterizes Cas12a-MG29-1, a metagenomics-derived nuclease, revealing structural and kinetic features that enhance its specificity and activity.

The paper compares Cas12f orthologs to identify mechanistic features that enhance genome editing efficiency in human cells.

The paper develops a biosensor to measure intracellular ADP-ribose dynamics and finds that ADPR levels are transient, tunable, and critical for TRPM2 activation.

This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.

This study defines the kinetic mechanism and rate constants for non-LTR retrotransposition using single-turnover analysis, revealing the complete reaction sequence and second-strand synthesis.

The paper develops a biosensor-AI system to engineer E. coli for efficient production of amaryllidaceae alkaloids, achieving improved titer and reduced byproducts.

This study investigates how SpRY-Cas9 recognizes DNA targets without requiring a PAM sequence, revealing structural and kinetic mechanisms that improve genome editing versatility.