Rebecca S. Shapiro

This study shows that the luminal domain of Ire1 in C. albicans is critical for cell wall integrity, thermal stress, and virulence, while the transmembrane domain is sufficient for azole antifungal tolerance.

This study uses CRISPR-activation to identify genes with fitness effects in Candida albicans isolates, revealing resistance genes and vulnerabilities associated with copy number variants.

The paper uses CRISPR screening to identify secretory pathway genes that drive antifungal tolerance through aneuploidy in Candida albicans.

This study identifies the Efg1 regulon active in C. albicans during growth in human urine, validates its clinical relevance in catheter samples, and characterizes key downstream factors involved in fungal CAUTI.

This study identifies Ire1-driven transcriptional responses distinct from the canonical UPR in C. albicans, revealing HAC1-independent pathways and RIDD activity in morphogenesis.

This study uses pooled CRISPRi screening in Candida albicans to identify dosage-sensitive genes across diverse environments and genetic backgrounds, highlighting potential antifungal targets.

This paper introduces a hyperdCas12a-based CRISPR system for multiplexed gene regulation in Candida albicans, demonstrating its effectiveness in studying genetic interactions and drug resistance.

The paper curates AMR mutations in fungi to create FungAMR, identifies universal resistance mutations, and provides a tool to screen genomes for AMR mutations.

This paper uses CRISPR-Cas9 to induce targeted loss of heterozygosity in Candida albicans and validates its role in antifungal resistance.

This study investigates the role of REV1, an error-prone polymerase, in DNA damage and antifungal drug resistance in Candida albicans, finding that its deletion increases mutation rates and resistance.

The paper evaluates 4,000 Erg11 variants to determine azole resistance and fitness costs, finding 33% resistance, 88% cross-resistance, and minimal fitness cost.

This paper develops and validates a CRISPR activation system in Nakaseomyces glabrata to study gene overexpression in pathogenesis and drug resistance.

The paper investigates how Tra1 regulates azole resistance in different yeast species, finding species-specific effects on gene expression and drug tolerance.