Kevin H. Gardner

This study identifies small molecules that bind to the PAS-A domain of CLOCK and NPAS2, stabilizing the domain and reducing DNA binding affinity of CLOCK:BMAL1.

This paper characterizes FG214, a heme-binding one-component switch from Fimbriimonas ginsengisoli, showing heme redox state controls monomer-dimer transitions and DNA binding.

This study uses circular protein-DNA chimeras to investigate conformational changes in ubiquitin induced by DNA attachment, showing that local DNA-protein interactions drive structural shifts.

This study investigates how context-dependent variability in HIF heterodimers affects their interactions with DNA, small molecules, and coactivators, revealing a novel 'dimer-of-heterodimers' structure.

The paper identifies small molecule ligands that stabilize a bacterial PAS-HTH transcription factor, CU228, using a combination of bioinformatics, thermal stability assays, and NMR.

This study shows blue light or high pressure activates AsLOV2 by expelling structured water, causing conformational changes and suggesting hydration water drives protein mechanics.

This study investigates the unique signaling mechanisms of the RT-HK kinase in the GSR network of Rubellimicrobium thermophilum, revealing inverted logic and specific downstream interactions.

The paper shows that proteins can dynamically encode environmental information through domain interactions, with pulsatory responses shaped by light, temperature, and evolution.

This study investigates how domain insertion in a synthetic enzyme fusion enables light-induced allosteric regulation through localized conformational changes and bidirectional domain coupling.

This paper develops and tests optogenetic tools in yeast to control phosphate-responsive gene expression using light-sensitive fusion proteins.

The paper identifies ligand-binding sites on and within the ARNT PAS-B domain using NMR, MD simulations, and ensemble docking, revealing distinct binding modes for three small molecules.

The paper improves the TAEL optogenetic system for zebrafish, enhancing gene expression induction while maintaining safety, and creates stable transgenic lines for broader use.