W. Lee Kraus

This study investigates PARP16's role in ovarian cancer by showing that inhibiting it with DB008 disrupts ribosome MARylation, impairs protein homeostasis, and reduces tumor growth.

This study uses CRISPRi Perturb-seq to analyze 1,408 autism risk genes in human cortical neurons, identifying 215 genes that cause global transcriptomic dysregulation and uncovering shared transcriptional pathways.

The paper identifies dynamic regulatory network hubs in human lineage specification using Perturb-Seq and constructs a deep learning model to predict perturbed TFs in patient transcriptomes.

The paper maps PARP1 ADP-ribosylated proteomes in breast cancer subtypes, identifying subtype-specific modifications and their functional implications.

This study benchmarks and optimizes Perturb-seq in human pluripotent stem cells, identifying shared regulatory networks during cardiomyocyte differentiation.

The paper examines the molecular effects of varying estradiol doses on estrogen receptor enhancers in breast cancer cells, finding distinct biological outcomes at low versus high doses.

This study shows that macrophage PARP1 depletion disrupts adipose tissue homeostasis by enhancing cell-cell crosstalk, leading to obesity and metabolic dysfunction.

This study identifies and characterizes 17 high frequency oncohistone mutations in breast cancer that promote cancer-related phenotypes and may act as potential cancer drivers.

This study identifies PARP7 as a key regulator of C/EBPβ-dependent adipogenesis and shows it senses nuclear NAD+ levels to control gene expression and lipid production in mammary glands.

This study identifies XCP, a protein from the lncRNA1456, which interacts with PHF8 to regulate gene expression and cancer pathways in breast cancer.

The paper identifies SERBP1 as a protein involved in PARylation-dependent complexes that regulate splicing, cell division, and ribosome biogenesis, with implications for neurodegenerative diseases.

This study identifies ASTER-B as a mediator of intracellular estradiol retention in breast cancer cells, which supports estrogen receptor-dependent gene expression and cell growth.

This study shows that relaxin reduces estrogen receptor alpha phosphorylation and alters estrogen-dependent gene expression in myometrial cells.

This study shows that RBN-2397, a PARP7 inhibitor, reduces ovarian cancer cell proliferation and migration, and synergizes with paclitaxel to enhance these effects.

The paper develops and characterizes new recombinant ADP-ribose binding reagents that enable species-specific detection of mono and poly ADP-ribose in antibody-based assays.

The paper shows that KAP1 negatively regulates RNA polymerase II elongation kinetics to enhance signal-induced transcription of Immediate Early Genes.

This paper identifies a PARP14/TARG1-regulated MARylation cycle that controls stress granule dynamics and translation in ovarian cancer cells, with potential therapeutic implications.

The paper develops and characterizes PAR-Trackers, new tools for detecting poly(ADP-ribose) in vitro and in vivo, showing improved sensitivity and spatial-temporal resolution.

The paper compares nascent transcription in primates to identify evolutionary patterns in coding and non-coding transcription, finding that transcription factor binding sites and adaptive substitutions drive species-specific differences.

The paper introduces dREG, a machine-learning method to identify active transcriptional regulatory elements from GRO-seq data, showing strong enrichment for functional marks.