Douglas A. Mitchell

This study characterizes two broadly reactive antibodies against a conserved cryptic epitope at the influenza HA head-stem interface, showing they are non-neutralizing and have limited protective efficacy.

The P450 ApyO crosslinks a 10-residue C-terminal peptide, and substitutions in the YLY motif switch the linkage among C−C, N−C and C−O bonds.

The paper identifies an enzymatic pathway for isoaspartate formation in bacterial ribosomal protein uS11, showing its conservation and structural importance.

This study uses molecular dynamics and deep learning to uncover the folding mechanisms of lasso peptides, revealing key thermodynamic and kinetic principles.

This study characterizes the biosynthesis of captophorins, a class of Cu-associated peptidic thiooxazole metallophores, by analyzing MNIO enzymes and their products.

This study shows lantibiotic-producing bacteria reduce microbiome resilience and increase susceptibility to infections after antibiotic treatment.

This study investigates the substrate specificity of the PEARL BhaBCAla, showing it can use non-cognate amino acids and tRNAs for peptide extension, with efficiency depending on both amino acid and tRNA features.

This study develops LassoESM, a language model tailored for lasso peptide property prediction, showing improved performance in substrate compatibility and biological activity prediction.

The paper reconstitutes daptide biosynthesis in vitro to reveal enzymatic requirements and demonstrates broad substrate tolerance for modifying peptide C-termini.

The paper characterizes ChlH, a flavin-dependent halogenase that halogenates internal and terminal tryptophan residues in diverse peptides and proteins, showing broad substrate promiscuity.

This paper investigates how DarE enzyme modifies DarA to form ether and C-C crosslinks, identifying key residues and mechanisms involved in radical-mediated biosynthesis.

The paper updates a genome mining tool to identify borosin precursor peptides and characterizes new biosynthetic features.

This study discovers a new class of macrocyclic peptides by mining genomes for biosynthetic gene clusters, revealing novel enzymatic modifications involving a cytochrome P450 and other enzymes.

The paper identifies lipoavitides, a novel class of RiPP/fatty acid hybrid lipopeptides with a unique HMP modification, and describes their biosynthetic pathway.

This study uses cell-free biosynthesis to produce lasso peptides, demonstrating their sequence diversity and enzyme-substrate tolerance.

RRE-Finder identifies RRE domains across RiPP biosynthetic gene clusters, discovering novel classes and providing a dataset for further biochemical study.

The paper mines over 100,000 genomes to identify 8,500 lanthipeptide precursor peptides, revealing their broader distribution and diversity across bacterial and archaeal phyla.