Ralph E. Kleiner

This study adapts RNABPP-PS to identify RNA-modifying enzymes in bacteria, revealing species-specific 5-methyluridine modification in B. subtilis 23S rRNA.

This study investigates the metabolism of epigenetic ribonucleosides in human cells, revealing cytotoxic effects and mechanisms of RNA misincorporation and nucleolar stress.

This paper develops a method for targeted RNA editing using a covalent adenosine deaminase-antisense oligo conjugate, achieving efficient and specific A-to-I editing with reduced off-target effects.

The paper investigates the effects of liver-specific Mettl14 deletion, finding it causes nuclear heterotypia and disrupts RNA export machinery.

This study identifies a minimal GU motif that directs hDUS2 to modify U20 in tRNA and develops a platform to screen inhibitors of hDUS2.

The paper introduces Induro-tRNAseq, a method using a high-processivity reverse transcriptase to profile tRNA modifications, revealing novel proline codon modifications in humans.

This study identifies DUS enzymes' role in tRNA modification and shows cell type-specific effects on translation.

This paper introduces SNIPER-seq, a method to map RNA structure dynamics with temporal resolution, revealing structural changes in human 5S rRNA during ribosome biogenesis.

This study shows oxaliplatin inhibits RNA polymerase I transcription in the nucleolus through a DNA damage signaling pathway distinct from conventional nucleolar DNA damage responses.