Michael P. Rout

This study develops multivalent anti-ACE2 nanobodies that broadly neutralize SARS-CoV-2 variants and sarbecoviruses with high potency and efficacy in animal models.

The paper constructs an integrative Brownian dynamics model of nucleocytoplasmic transport, revealing how FG repeat proteins and nuclear transport receptors mediate selective transport through NPCs.

The paper identifies a novel phosphorylated NES in Pho4 recognized by Msn5, revealing a phosphate-specific nuclear export mechanism in yeast.

This study identifies mechanisms enabling nanobodies to bind antigens with high affinity and stability, revealing a conserved region critical for their function and suggesting strategies for their optimization.

This study uses structural biology to reveal the architecture of the nuclear basket in yeast and mammals, showing how Mlp/Tpr proteins form its structural components.

This paper develops and characterizes 50 nm diameter genetically encoded multimeric nanoparticles (GEMs) for improved nanorheology studies.

The paper uses site-specific deuteration and SANS to study the dynamic structure of FG Nups interacting with NTF2, revealing their fully disordered nature and lack of significant inter-aromatic contacts.

The paper identifies Crm1 as an adaptor that links transcription factors to chromatin at the nuclear pore complex, facilitating enhancer docking.

This paper introduces CTCC, a method using cryomilling to map chromatin interactions, showing it can generate global contact maps similar to Hi-C but with enhanced signal for stable inter-chromosomal contacts.

The paper identifies distinct nuclear and cytoplasmic intermediates of LINE-1 retrotransposition using mass spectrometry and affinity purification.

This paper introduces a rapid method for isolating well-preserved yeast nuclei, enabling detailed structural and functional studies.