Liam J. Holt

This study shows that mTOR signalling, not clock gene rhythms, drives daily physiological variations in mammals and plants.

The paper designs functional disordered regions in a transcription factor using biophysical principles to predict chromatin binding function.

ZATELLITE enables real-time visualization and manipulation of human centromeres in live cells using synthetic zinc fingers without causing DNA damage.

This study shows that pro-inflammatory polarization of macrophages increases nuclear stiffness despite reduced lamin A/C levels, suggesting chromatin changes drive nuclear mechanics.

The paper shows that increasing translation initiation rates accelerates nucleation of aggregating proteins at polysomes, suggesting cotranslational nucleation influences proteome evolution.

This study shows HSV-1 protein ICP4 increases nuclear fluidity to enable condensate formation, supporting viral replication.

This study shows that quiescence in Candida albicans enhances survival during antifungal drug exposure by altering cellular properties and gene expression.

This study examines how HSV-1 ICP4 expression in uninfected cells alters host gene expression through RNA-Seq, identifying differentially expressed genes and validating findings against infected cell data.

This study identifies the A-type lamin rod domain as the key determinant of nuclear deformability, revealing its unique role in resisting large mechanical forces on the nucleus.

This study tracks ribosome-sized particles in E. coli to show that their 3D diffusion is influenced by macromolecular interactions and geometrical confinement, revealing sub-diffusive motion due to nucleoid and cell boundary constraints.

The paper identifies two conserved mechanisms controlling cytoplasmic macromolecular diffusion in C. elegans tissues, involving ribosome concentration and the giant KASH protein ANC-1.

The paper identifies 68 yeast genes that globally influence protein phase separation through genetic knockouts and synthetic biology.

This paper develops and characterizes 50 nm diameter genetically encoded multimeric nanoparticles (GEMs) for improved nanorheology studies.

KaryoTap enables high-throughput single-cell aneuploidy profiling, revealing that positive and negative selection shape cancer genomes by influencing chromosomal gains and losses.

The paper shows that the SWI/SNF complex uses a pH-sensitive low complexity sequence to sense carbon starvation in yeast.

The paper shows synthetic condensates enhance kinase activity and specificity, revealing principles of phosphorylation dynamics in response to macromolecular crowding.

The paper uses synthetic genomic reconstitution to study Hox cluster regulation, finding that distal enhancers are required for full transcriptional output while chromatin remodeling can occur without them.

The paper identifies functionally important regions in ORF1p and ORF2p of human LINE-1 through trialanine mutagenesis, showing most mutants lose retrotransposition activity.

The paper uses ancestral resurrection to show that changes in the β3-C loop and gatekeeper residue in ERK kinases drove the evolution of lower intrinsic autophosphorylation.

This study shows that phosphoregulation of the mitotic kinesin-5 Cin8 requires site-specific and precise phosphorylation sites to achieve optimal regulation.

The paper shows that a polyglutamine domain in the yeast SWI/SNF complex functions as a pH sensor for transcriptional regulation during carbon starvation.