Brian T. Chait

This study develops multivalent anti-ACE2 nanobodies that broadly neutralize SARS-CoV-2 variants and sarbecoviruses with high potency and efficacy in animal models.

This paper introduces a nature-inspired ion trap that enables massively parallel mass spectrometry, improving signal-to-noise ratios and detection sensitivity through parallel ion manipulation.

This study reveals the structural basis and evolutionary origin of the Lamassu bacterial immune system, showing its adaptation from DNA repair machinery to detect and respond to viral replication.

This paper investigates the mechanisms of helicase loading and translocation by the Vibrio cholerae DnaB helicase and DciA loader, revealing structural and biochemical insights into their interactions.

This study identifies mechanisms enabling nanobodies to bind antigens with high affinity and stability, revealing a conserved region critical for their function and suggesting strategies for their optimization.

This study identifies how VCP interacts with DUBs at distinct sites to coordinate ubiquitin removal during protein recycling.

This paper develops targeted mass spectrometry assays to detect and quantify endogenous ORF1p and ORF2p proteins, demonstrating sensitive and robust quantitation of ORF2p for the first time.

The paper shows that KorB, a DNA-sliding clamp, switches to a repressor role with KorA to mediate long-range gene silencing on plasmid RK2.

The paper shows RapA disrupts post-termination complexes in E. coli to prevent R-loop formation and maintain genome stability.

This study uses structural biology to reveal the architecture of the nuclear basket in yeast and mammals, showing how Mlp/Tpr proteins form its structural components.

The paper develops covalent inhibitors for conformationally dynamic helicases using electrophilic scout fragments and a function-first approach, targeting SARS-CoV-2 nsp13 and human helicases BLM and WRN.

This study determines cryo-EM structures of SARS-CoV-2 NiRAN domain catalytic intermediates, revealing structural mechanisms for NMPylation and deRNAylation/capping, and explaining substrate preference for capping.

This study identifies phosphorylation of RIF1's intrinsically disordered region as essential for protecting nascent DNA at stalled replication forks during replication stress.

The paper identifies distinct nuclear and cytoplasmic intermediates of LINE-1 retrotransposition using mass spectrometry and affinity purification.