James Barrett

This study characterizes the pyrenoid structure and molecular components of Chlorella sorokiniana, revealing conserved inorganic carbon delivery machinery and novel proteins involved in pyrenoid assembly and starch tethering.

Fluorescent tagging of 160 proteins in the seaweed Ulva maps its carbon-concentrating pathway, and identifies SUPA1, a Rubisco linker that binds only four of eight sites.

This study shows that varying the number of binding motifs in a disordered protein affects pyrenoid assembly and algal fitness.

This study investigates the role of LCI9 in starch sheath formation and CO2-concentrating mechanism efficiency in Chlamydomonas reinhardtii, finding that LCI9 is essential for proper sheath assembly and pCCM function.

The paper identifies a conserved linker protein from Chlorella that can phase separate Rubisco in Chlamydomonas and some plants, enabling cross-species pyrenoid formation.

The paper identifies a protein interaction network in the diatom pyrenoid, revealing a structural shell critical for CO2 fixation and suggesting convergent evolution with prokaryotic carboxysomes.

This study establishes Chlamydomonas reinhardtii as a platform for chloroplast synthetic biology, enabling high-throughput engineering of plastomes and demonstrating improved biomass production through synthetic pathways.