Xavier Darzacq

The paper uses an arrayed CRISPR screen to identify ZBTB10 as a regulator of human trophoblast differentiation, showing its role in fusion and hormone secretion.

This study shows that increasing MyoD1 levels in myoblasts expands its genome binding and upregulates cell adhesion genes, leading to impaired fusion but enhanced myotube contraction.

This study shows that short activation domains, not just DNA-binding domains, significantly influence chromatin association of transcription factors in living cells.

This study uses PAPA-fSMT to investigate P-TEFb interactions and exchange in live cells, revealing dynamics of the 7SK ribonucleoprotein complex and its association with chromatin and BRD4.

This paper introduces Oblique Line Scan (OLS) illumination to enhance single-molecule tracking in living cells and solution, enabling high-throughput and sensitive protein dynamics measurements.

The paper identifies HR factors that inhibit dual AAV vector transduction and shows that inhibiting these factors improves transgene reconstitution.

The paper uses automated live-cell single-molecule tracking to study transcription factor dynamics in mouse enteroid monolayers, revealing contrasting diffusive behaviors that correlate with differentiation stages.

This study identifies TFEB as a transcriptional regulator of syncytiotrophoblast differentiation by controlling Syncytin-1 and Syncytin-2 expression in BeWo cells.

The paper uses live cell imaging to show that RAR, not RXR, limits heterodimerization and chromatin binding of T2NRs, challenging previous assumptions about RXR's role.

The paper shows that RNA polymerase II depletion from the inactive X chromosome is due to reduced chromatin binding, not biophysical compartmentalization.

The paper investigates how HIF-1 and HIF-2 subunits exhibit distinct binding dynamics and gene regulation despite sharing the same DNA binding specificity, revealing the role of intrinsically disordered regions in chromatin binding and target selectivity.

This study investigates how tuning low-complexity domain interactions in the oncogenic TF EWS/FLI1 affects transcription and oncogenic transformation in human cells.

This paper introduces a Bayesian nonparametric framework for identifying individual molecules in super-resolution microscopy time series, using ground-truth data to assess performance.

The paper investigates how enhancer-promoter interactions and transcription are affected by acute loss of CTCF, cohesin, WAPL, and YY1, finding minimal effects on E-P interactions and gene expression despite 3D genome perturbations.

The paper shows cohesin residency at CARs determines chromatin loop positioning in budding yeast through loop extrusion and CAR-bound cohesin stopping.

The paper investigates how transcriptional regulatory elements influence the 3D organization of microTADs in mouse stem cells.

The paper shows that herpesvirus creates replication compartments that recruit RNA Polymerase II through DNA interactions, not phase separation, leading to local protein accumulations.

The paper identifies an integrated gene repression mechanism in human cells through mRNA isoform toggling at the MDM2 locus, similar to yeast.

The paper shows CTCF binding and nucleosome positioning at CTCF sites change during the cell cycle, with loss of binding in prometaphase and rearrangement of chromatin structure.

This study shows that transcription factors like Zelda and Bicoid form dynamic, transient hubs in Drosophila embryos that interact with active transcription sites.

This study shows that RNA polymerase II CTDs undergo phase separation, forming clusters that are regulated by phosphorylation and CTD length.

The paper shows TBP remains bound to promoters during mitosis, stabilizing RNA Polymerase II association and enabling transcriptional reactivation.

The paper investigates how Wnt-responsive enhancers selectively activate alternative MYC promoters, showing their role in differential promoter usage and the influence of core promoter elements.

This study uses live-cell single-molecule imaging to show that TF low-complexity domains form dynamic, selective interaction hubs that stabilize DNA binding and activate transcription.

The paper uses live imaging in Drosophila embryos to show that Bicoid binds DNA with a rapid off-rate and forms localized hubs that enable concentration-sensitive regulation.

The paper shows that many transcription factors remain bound to mitotic chromosomes, challenging previous findings of their exclusion and revealing dynamic interactions that depend on DNA binding and nuclear import.