Johannes Broichhagen

This study shows how transient secondary structure in intrinsically disordered regions can generate new molecular functions while preserving ancestral activities in paralogous proteins.

This study shows that spreading tau coordinates lipid homeostasis by promoting neuronal lipid efflux and enhancing resilience to lipid toxicity.

The paper designs a binary fluorescent turn-on probe using a HaloTag mutant and a difluorinated xanthene ligand for covalent labeling and imaging applications.

The paper introduces a new protein labelling technology called CLUSTER that enables efficient and minimally invasive protein labeling in living cells using a bio-orthogonal approach.

This study investigates the molecular interactions between KCNQ1, KCNE1, and BACE1 using multiple assays, revealing domain-specific contributions and functional additivity.

The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.

This study identifies shared molecular mechanisms by which MRAP2 regulates GPCR signaling and shows that obesity-associated variants disrupt these mechanisms.

The paper introduces a one-step method to create cell-impermeable fluorescent HaloTag substrates for live-cell imaging and super-resolution microscopy.

This study shows MRAP2 interacts with MC3R to enhance its signaling, affecting energy homeostasis and obesity risk.

The paper identifies the melanocortin system's role in heightened carotid body chemosensitivity in hypertension, showing MC4R activation influences sympathetic and ventilatory responses.

This paper introduces a knock-in mouse line with endogenous Munc13-1-SNAP for monitoring presynaptic nanoarchitecture using SNAP-tag labeling and live-cell imaging.

This paper develops a fluorescent probe to detect malaria-infected red blood cells and demonstrates its use with a smartphone microscope for diagnostic applications.

This study investigates the structural and dynamic diversity of metabotropic glutamate receptor/beta-arrestin coupling using biophysical and structural methods, revealing new coupling stoichiometries and interaction mechanisms.

This paper develops peptide-coated nanocapsules for targeted drug delivery to pancreatic β-cells, showing effective in vitro and in vivo targeting and cargo delivery.

This paper introduces deuterated azobenzene photoswitches to enhance photopharmacological molecules by improving light sensitivity, photoswitch efficiency, and kinetics.

The paper uses photopharmacology to show that mGluR2 in specific brain projections regulates distinct anxiety-related behaviors.

This paper introduces a method for saturated reconstruction of living brain tissue using advanced imaging and deep learning to achieve high-resolution, volumetric analysis of neural structures over time.

The paper introduces real-time library search (RTLS) to improve cross-link identification in XL-MS by leveraging intensity ratios of diagnostic peaks, enhancing identification depth in complex samples.

This paper demonstrates a chemical-optogenetic approach that restores high-sensitivity motion vision in an IRD mouse model using a multi-branched photoswitch.

This study identifies subtype-specific differences in transmembrane domain interactions that influence metabotropic glutamate receptor activation.