Max Ruwolt

This study identifies structural elements and dynamics of the Mic60-Mic19 subcomplex crucial for MICOS assembly and crista junction formation.

The paper develops a mass spectrometry method to quantify inositol pyrophosphate-protein interactions on a proteome-wide scale, identifying new pyrophosphorylated proteins and highlighting Mg2+ effects on binding.

The paper identifies mTOR's role in regulating developmental gene promoters in pluripotent mouse embryonic stem cells through interaction with PRC1 and RNA Polymerase II pausing.

This study shows MTMR14 is recruited to damaged lysosomes to remodel lipids, reduce mTORC1 signaling, and support proteostasis during lysosomal injury.

The paper uses integrative structural biology to model the human Mic60-Mic19 subcomplex and validate its role as a diffusion barrier in mitochondrial crista junctions.

This study shows that dietary lipids induce caveolae uptake, caveolin1 accumulation to lipid droplets, and caveolin1-dependent lipid droplet growth.

This study identifies PLPPR3 condensates as actin sinks that locally boost monomer concentrations, promoting actin polymerization and filopodia formation in neurons.

This study identifies oligophosphorylation as a new phosphorylation mode, showing NME1 can catalyze its own oligophosphorylation using ATP, with implications for phosphoregulation.

This study uses cryo-electron tomography to describe the molecular architecture and structural heterogeneity of individual synaptic vesicles in mouse brains and cultured neurons.

The paper develops recombinant protein standards and a search engine for XL-MS, showing Scout outperforms existing tools in speed, sensitivity, and false-discovery rate control.

This study identifies mitochondrial adaptations in naked mole-rats that confer hypoxia resistance, linking social behavior to molecular evolution.

This paper introduces CLASP, a cross-link assisted spatial proteomics method, to map sub-organelle proteomes and membrane protein topology in human mitochondria.

This study compares four lysis buffers for proteomics, finding SDS most reliable and advocating a dual-sample approach to maximize proteome coverage.

The paper improves inter-link detection in cross-linking mass spectrometry by using a fused database and context-sensitive subgrouping, increasing coverage by 30-75% while maintaining low error rates.

The paper introduces real-time library search (RTLS) to improve cross-link identification in XL-MS by leveraging intensity ratios of diagnostic peaks, enhancing identification depth in complex samples.