Matthew S. Bogyo

This paper develops a two-step synthesis method for covalent genetically-encoded libraries of peptide-derived macrocycles (cGELs) that enables the use of electrophiles with diverse reactivity by controlling reaction conditions.

This paper identifies a covalent irreversible inhibitor, Z34, that binds to ALDH1A3 in two distinct conformations at the active-site cysteine, showing promise for anticancer drug development.

This study develops a covalent probe for imaging S. aureus biofilms in vitro and in vivo, showing high specificity for the serine hydrolase FphE.

This study shows that broad-spectrum cathepsin inhibitors block crystal-induced inflammation and gout arthritis by inhibiting both inflammasome-dependent and -independent pathways that generate IL-1β.

The paper characterizes the structural and ligand-binding properties of Staphylococcus aureus FphE, revealing its unique dimeric structure and substrate engagement mechanisms.

The paper designs a caspase-1-cathepsin AND-gate probe to selectively image inflammasome activation by requiring processing by both enzymes.

This paper develops a recombinant Pf20S platform for structural analysis and inhibitor design, identifying a selective inhibitor with high potency against Plasmodium falciparum proteasome.

This paper introduces a method using macrocyclic phage display to identify selective protease substrates, demonstrating its effectiveness with FAP and DPP4.

The paper develops fluorescent probes for FphB, a Staphylococcus aureus enzyme, using mRNA display with a genetically encoded electrophile, achieving nanomolar selectivity.

This paper identifies covalent cyclic peptide inhibitors targeting protein-protein interactions using phage display, demonstrating antiviral activity against SARS-CoV-2.

This paper develops a fluorogenic substrate for Chikungunya virus protease (nsP2) that improves signal-to-noise ratio and specificity for inhibitor screening.

This study identifies a secreted protease from Bacteroides fragilis that cleaves PAR2, leading to intestinal inflammation and pain in mouse models.

The paper identifies mixed alkyl/aryl phosphonates as anti-malarial compounds that inhibit serine hydrolases with low resistance potential.

This paper develops polymer-tethered fluorescent probes for tumor-associated protease imaging, showing improved signal and tumor-to-background ratios compared to small molecule probes.

The paper develops oxadiazolone-based probes targeting FphE to specifically detect S. aureus infections through covalent modification of the enzyme's active site.