John F. Marko

The paper demonstrates that mitotic chromosomes are mechanically connected by DNA-based interchromosome linkers, identified through micropipette manipulation and enzymatic treatments.

This study shows the segment-capture model of SMC complexes can explain obstacle bypassing and loop extrusion, and proposes experiments to test the model.

The paper develops a theory to predict how molecular crowding affects the extension of stretched DNA under tension, showing both compression and expansion effects depending on crowder density and size.

This study investigates GapR's role in DNA mechanics and transcription regulation using biophysical and computational methods, revealing its dual effects on DNA structure and transcription.

This study investigates how molecular crowding and dehydrating co-solutes affect DNA supercoiling and base-pair stability using single molecule magnetic tweezers.

This study shows that centromeric chromatin is mechanically resilient and distinct from pericentromeric chromatin, which deforms under force, with CCAN components not affecting pericentromere stretching.

This study isolates meiotic spindles from mouse oocytes to investigate migration forces during asymmetric division, finding a net tensile force of ~680 pN.

This study measures chromosome stiffness in mouse oocytes across cell cycle stages and age, finding increased stiffness in metaphase I oocytes and age-related stiffness changes.

The paper introduces GapR-seq, a method to map positive supercoiling in genomes, and finds it widespread, associated with transcription and specific genomic features in E. coli and yeast.

This study introduces liquid chromatin Hi-C to analyze chromatin interaction dynamics and finds that compartmentalization stability varies with chromatin domain type and fragment size.

This study shows condensin controls mitotic chromosome stiffness and stability by forming isolated compaction centers rather than a continuous scaffold.

This study demonstrates a method using magnetic tweezers to detect single base pair mismatches in DNA by observing plectoneme pinning under specific conditions.