Rapid, one-step generation of biallelic conditional gene knockouts

Loss-of-function studies are key to investigate gene function and CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional gene knockouts in diploid cells by co-delivery of CRISPR/Cas9 and a universal conditional intron cassette.