A mutant of the phototoxic protein KillerRed that does not form DsRed-like chromophore
Abstract
We report a mutant of the phototoxic fluorescent protein KillerRed protein with fluorescence in the green part of the spectrum. The mutant variant carries mutations I64L, D114G, and T115S and does not form a DsRed-like chromophore. The protein can be used as a template to create new genetically encodable photosensitizers that are spectrally different from KillerRed.
02 /Specification & Classification
Click any tag to browse matching papers- Assay
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Fluorogenesis: Inducing Fluorescence in a Non-Fluorescent Protein Through Photoinduced Chromophore Transfer of a Genetically Encoded Chromophore
Y. Kumar, R. K. Singh, … K. Pushpavanam·bioRxiv·Jun 24, 2023
- Structure-guided point mutations on FusionRed produce a brighter red fluorescent protein
S. Mukherjee, S. Hung, … R. Jimenez·bioRxiv·Apr 20, 2020
- Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry
T. Y. H. Bui, E. De Zitter, … B. Pedre·bioRxiv·Jan 13, 2023
- Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.
J. O. Onukwufor, A. J. Trewin, … A. P. Wojtovich·bioRxiv·Sep 20, 2019
- Integrated structure-function dataset reveals key mechanisms underlying photochromic fluorescent proteins
E. De Zitter, S. Hugelier, … P. Dedecker·bioRxiv·Sep 25, 2020
- Characterization of fluorescent proteins with intramolecular photostabilization
S. Henrikus, K. Tassis, … T. Cordes·bioRxiv·Mar 7, 2020